Heat-induced formation of single giant unilamellar vesicles
Artikel i vetenskaplig tidskrift, 2011

Giant unilamellar vesicles (GUVs) are an excellent model system for the investigation of lipid membranes, the study of membrane proteins and ion channels in a biomimetic environment, and in the creation of artificial cells. Here, we describe a novel method for the preparation of GUVs from single multilamellar liposomes by means of directed infrared laser heating. Our method generates individual unilamellar vesicles at selected locations, not only from natural and artificial lipid mixtures containing negatively charged lipids, but also from preparations of single lipids, such as neutral phosphatidylethanolamine. The presented method provides a new efficient resource for giant vesicle research and offers an alternative to the electroformation and de/rehydration techniques.

Liposomes

Giant Unilamellar Vesicles

Heat-induced formation

Multilamellar Vesicles

Nanotube-Vesicle Networks

Författare

Celine Billerit

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Ilona Wegrzyn

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Gavin Jeffries

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Paul Gunnar Dommersnes

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Owe Orwar

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Aldo Jesorka

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Soft Matter

1744-683X (ISSN) 1744-6848 (eISSN)

Vol. 7 20 9751-9757

Ämneskategorier

Fysikalisk kemi

DOI

10.1039/c1sm05444a