Mapping the interaction of Snf1 with TORC1 in Saccharomyces cerevisiae
Artikel i vetenskaplig tidskrift, 2011

Nutrient sensing and coordination of metabolic pathways are crucial functions for all living cells, but details of the coordination under different environmental conditions remain elusive. We therefore undertook a systems biology approach to investigate the interactions between the Snf1 and the target of rapamycin complex 1 (TORC1) in Saccharomyces cerevisiae. We show that Snf1 regulates a much broader range of biological processes compared with TORC1 under both glucose-and ammonium-limited conditions. We also find that Snf1 has a role in upregulating the NADP(+)-dependent glutamate dehydrogenase (encoded by GDH3) under derepressing condition, and therefore may also have a role in ammonium assimilation and amino-acid biosynthesis, which can be considered as a convergence of Snf1 and TORC1 pathways. In addition to the accepted role of Snf1 in regulating fatty acid (FA) metabolism, we show that TORC1 also regulates FA metabolism, likely through modulating the peroxisome and beta-oxidation. Finally, we conclude that direct interactions between Snf1 and TORC1 pathways are unlikely under nutrient-limited conditions and propose that TORC1 is repressed in a manner that is independent of Snf1.



yeast snf1

nutrient sensing

carbon metabolism

signaling pathways


glucose repression



nitrogen metabolism


functional genomics

cell-growth control

chemical genomics

activated protein-kinase


Jie Zhang

Chalmers, Kemi- och bioteknik, Livsvetenskaper

S. Vaga

Eidgenössische Technische Hochschule Zürich (ETH)

Pramote Chumnanpuen

Chalmers, Kemi- och bioteknik, Livsvetenskaper

Rahul Kumar

Chalmers, Kemi- och bioteknik, Livsvetenskaper

Goutham Vemuri

Chalmers, Kemi- och bioteknik, Livsvetenskaper

R. Aebersold

Universität Zürich

Eidgenössische Technische Hochschule Zürich (ETH)

Jens B Nielsen

Chalmers, Kemi- och bioteknik, Livsvetenskaper

Molecular Systems Biology

17444292 (eISSN)

Vol. 7 545


Biokemi och molekylärbiologi


Grundläggande vetenskaper


Livsvetenskaper och teknik (2010-2018)



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