Microarrays Made Easy: Biofunctionalized Hydrogel Channels for Rapid Protein Microarray Production
Artikel i vetenskaplig tidskrift, 2011

We present a simple, inexpensive, and sensitive technique for producing multiple copies of a hydrogel-based protein microarray. An agarose block containing 25 biofunctionalized channels is sliced perpendicularly to produce many identical biochips. Each microarray consists of 500 mu m spots, which contain protein-coated microparticles physically trapped in porous SeaPrep agarose. Proteins diffuse readily through SeaPrep agarose while the larger microparticles are immobilized in the hydrogel matrix Without major assay optimization the limit of detection is 12 pM for a sandwich assay detecting human IgG. These highly flexible, multiplexed arrays can produced rapidly without any special instrumentation and are compatible with standard fluorescence-based read-out.

protein microarray

agarose

microparticle-based assay

hydrogel

microchannel

Författare

V. de Lange

A. Binkert

J. Vörös

Marta Bally

Chalmers, Teknisk fysik, Biologisk fysik

ACS Applied Materials & Interfaces

1944-8244 (ISSN) 1944-8252 (eISSN)

Vol. 3 1 50-57

Ämneskategorier

Materialteknik

Medicinsk laboratorie- och mätteknik

DOI

10.1021/am100849f