High-Resolution Micropatterned Teflon AF Substrates for Biocompatible Nanofluidic Devices
Artikel i vetenskaplig tidskrift, 2012

We describe a general photolithography-based process for the microfabrication of surface-supported Teflon AF structures. Teflon AF patterns primarily benefit from superior optical properties such as very low autofluorescence and a low refractive index. The process ensures that the Teflon AF patterns remain strongly hydrophobic in order to allow rapid lipid monolayer spreading and generates a characteristic edge morphology which assists directed cell growth along the structured surfaces. We provide application examples, demonstrating the well-controlled mixing of lipid films on Teflon AF structures and showing how the patterned surfaces can be used as biocompatible growth-directing substrates for cell culture. Chinese hamster ovary (CHO) cells develop in a guided fashion along the sides of the microstructures, selectively avoiding to grow over the patterned areas.

films

surfaces

lipid-bilayers

membranes

growth

planar

cells

area

adhesion

Författare

Ilja Czolkos

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Bodil Hakonen

Chalmers, Kemi- och bioteknik, Kärnkemi

Owe Orwar

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Aldo Jesorka

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Langmuir

0743-7463 (ISSN) 1520-5827 (eISSN)

Vol. 28 6 3200-3205

Ämneskategorier

Kemi

DOI

10.1021/la2044784