Structure Dynamics and Heterogeneity in Soft Materials Determined by FRAP
Doktorsavhandling, 2012

A common interest in industry today is to be able to control the mass transport inside soft (bio) materials in order to tailor both release and uptake of substances. Most of the materials used are heterogeneous, quite often multiphase or compound materials and often undergo dynamic changes; it is therefore important to have control over the local microstructure in order to tailor the macroscale properties. The work in this thesis expands the toolbox of available techniques for studying diffusion in such materials. This has been achieved by enhancing the usability of a technique called fluorescence recovery after photobleaching (FRAP) as well as by performing FRAP measurements in dynamic and heterogeneous materials. FRAP is an optical technique capable of measuring locally at a micrometer scale in a material sample. The results obtained have given an increased understanding of the structure–mass transport relationship. The technique has been improved by improving the accuracy of the mass transport determination, by reducing the required measurement area, by increasing the range of available materials in which FRAP can be used as well as by analysing the impact of probe selection. The materials used in this thesis work have been used as model materials for FRAP development and at the same time been investigated using FRAP. The materials, along with the key interest, can be summarized to the following choises: κ-carrageenan for the structural heterogeneity, gelatin for the dynamic changes during gelation, gelatin/maltodextrin multiphase samples for the posibillty to tailor the domain sizes, super absorbing polymers (SAP) for the posibillity to alter the mass transport properties depending on the swelling and both β-lactoglobulin and pasta was used as model material for spatial correlation measurements of FRAP data. These results may be used for improved understanding of other materials as well and the FRAP technique is shown to be one promising tool when tailoring new structures.

Fluorescence recovery after photobelaching

Structure dynamics

Soft materials


Confocal microscopy

KC, Kemigården 4, Chalmers tekniska högskola
Opponent: Prof. Taco Nicolai, Department of Polymères, Colloïdes, Interfaces, Université du Maine, France


Joel H Hagman

SuMo Biomaterials

Chalmers, Kemi- och bioteknik, Teknisk ytkemi

Straightforward FRAP for quantitative diffusion measurements with a laser scanning microscope

Optics Express,; Vol. 18(2010)p. 22886-22905

Artikel i vetenskaplig tidskrift

Effect of Gelatin Gelation Kinetics on Probe Diffusion Determined by FRAP and Rheology

Biomacromolecules,; Vol. 11(2010)p. 3359-3366

Artikel i vetenskaplig tidskrift

Pixel-based analysis of FRAP data with a general initial bleaching profile

Journal of Microscopy,; Vol. 239(2010)p. 142-153

Artikel i vetenskaplig tidskrift

Probe diffusion in κ-carrageenan gels determined by fluorescence recovery after photobleaching

Food Hydrocolloids,; Vol. 29(2012)p. 106-115

Artikel i vetenskaplig tidskrift





Livsvetenskaper och teknik




Doktorsavhandlingar vid Chalmers tekniska högskola. Ny serie: 3364

KC, Kemigården 4, Chalmers tekniska högskola

Opponent: Prof. Taco Nicolai, Department of Polymères, Colloïdes, Interfaces, Université du Maine, France