Radial Sizing of Lipid Nanotubes Using Membrane Displacement Analysis
Artikel i vetenskaplig tidskrift, 2012
We report a novel method for the measurement of lipid nanotube radii. Membrane translocation is monitored between two nanotube-connected vesicles, during the expansion of a receiving vesicle, by observing a photobleached region of the nanotube. We elucidate nanotube radii, extracted from SPE vesicles, enabling quantification of membrane composition and lamellarity. Variances of nanotube radii were measured, showing a growth of 40-56 nm, upon increasing cholesterol content from 0 to 20%.
membrane displacement
liposomes
target-cells
transmission
intracellular trafficking
cholesterol
depletion
transport
cholesterol
vesicle
microscopy
proteins
plasma-membrane
Lipid nanotube
Författare
Natalia Stepanyants
Chalmers, Kemi- och bioteknik, Fysikalisk kemi
Gavin Jeffries
Chalmers, Kemi- och bioteknik, Fysikalisk kemi
Owe Orwar
Chalmers, Kemi- och bioteknik, Fysikalisk kemi
Aldo Jesorka
Chalmers, Kemi- och bioteknik, Fysikalisk kemi
Nano Letters
1530-6984 (ISSN) 1530-6992 (eISSN)
Vol. 12 3 1372-1378Ämneskategorier
Kemi
DOI
10.1021/nl203983e