Radial Sizing of Lipid Nanotubes Using Membrane Displacement Analysis
Artikel i vetenskaplig tidskrift, 2012

We report a novel method for the measurement of lipid nanotube radii. Membrane translocation is monitored between two nanotube-connected vesicles, during the expansion of a receiving vesicle, by observing a photobleached region of the nanotube. We elucidate nanotube radii, extracted from SPE vesicles, enabling quantification of membrane composition and lamellarity. Variances of nanotube radii were measured, showing a growth of 40-56 nm, upon increasing cholesterol content from 0 to 20%.

membrane displacement

liposomes

target-cells

transmission

intracellular trafficking

cholesterol

depletion

transport

cholesterol

vesicle

microscopy

proteins

plasma-membrane

Lipid nanotube

Författare

Natalia Stepanyants

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Gavin Jeffries

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Owe Orwar

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Aldo Jesorka

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Nano Letters

1530-6984 (ISSN) 1530-6992 (eISSN)

Vol. 12 3 1372-1378

Ämneskategorier

Kemi

DOI

10.1021/nl203983e