De novo sequencing, assembly and analysis of the genome of the laboratory strain Saccharomyces cerevisiae CEN.PK113-7D, a model for modern industrial biotechnology
Artikel i vetenskaplig tidskrift, 2012
Saccharomyces cerevisiae CEN.PK 113-7D is widely used for metabolic engineering and systems biology research in industry and academia. We sequenced, assembled, annotated and analyzed its genome. Single-nucleotide variations (SNV), insertions/deletions (indels) and differences in genome organization compared to the reference strain S. cerevisiae S288C were analyzed. In addition to a few large deletions and duplications, nearly 3000 indels were identified in the CEN.PK113-7D genome relative to S288C. These differences were overrepresented in genes whose functions are related to transcriptional regulation and chromatin remodelling. Some of these variations were caused by unstable tandem repeats, suggesting an innate evolvability of the corresponding genes. Besides a previously characterized mutation in adenylate cyclase, the CEN. PK113-7D genome sequence revealed a significant enrichment of non-synonymous mutations in genes encoding for components of the cAMP signalling pathway. Some phenotypic characteristics of the CEN. PK113-7D strains were explained by the presence of additional specific metabolic genes relative to S288C. In particular, the presence of the BIO1 and BIO6 genes correlated with a biotin prototrophy of CEN. PK113-7D. Furthermore, the copy number, chromosomal location and sequences of the MAL loci were resolved. The assembled sequence reveals that CEN. PK113-7D has a mosaic genome that combines characteristics of laboratory strains and wild-industrial strains.
yeast genome
protein
chemostat
gene prediction
alcoholic fermentation
l-arabinose
cultures
evolutionary
xylose
glucose
biotin-prototrophy
Författare
J. F. Nijkamp
TU Delft
Kluyver Centre for Genomics of Industrial Fermentation
M. van den Broek
Kluyver Centre for Genomics of Industrial Fermentation
TU Delft
E. Datema
Wageningen University and Research
Centre for Biosystems Genomics, Wageningen
Keygene N.V.
S. de Kok
Amyris, Inc.
Kluyver Centre for Genomics of Industrial Fermentation
TU Delft
L. Bosman
Kluyver Centre for Genomics of Industrial Fermentation
TU Delft
M. A. Luttik
TU Delft
Kluyver Centre for Genomics of Industrial Fermentation
P. Daran-Lapujade
TU Delft
Kluyver Centre for Genomics of Industrial Fermentation
Wanwipa Vongsangnak
Chalmers, Kemi- och bioteknik, Livsvetenskaper
Jens B Nielsen
Chalmers, Kemi- och bioteknik, Livsvetenskaper
W. H. M. Heijne
DSM Biotechnology Centre
P. Klaassen
DSM Biotechnology Centre
C. J. Paddon
Amyris, Inc.
D. Platt
Amyris, Inc.
P. Kotter
Johann Wolfgang Goethe Universität Frankfurt am Main
R. C. van Ham
Keygene N.V.
Wageningen University and Research
Centre for Biosystems Genomics, Wageningen
M. J. T. Reinders
TU Delft
Netherlands Bioinformatics Centre - NBIC
Kluyver Centre for Genomics of Industrial Fermentation
J. Pronk
TU Delft
Kluyver Centre for Genomics of Industrial Fermentation
D. De Ridder
Netherlands Bioinformatics Centre - NBIC
Platform for Green Synthetic Biology
TU Delft
Kluyver Centre for Genomics of Industrial Fermentation
J. M. Daran
TU Delft
Platform for Green Synthetic Biology
Kluyver Centre for Genomics of Industrial Fermentation
Microbial Cell Factories
14752859 (eISSN)
Vol. 11 Article Number: 36- 36Ämneskategorier
Industriell bioteknik
Styrkeområden
Livsvetenskaper och teknik (2010-2018)
DOI
10.1186/1475-2859-11-36