Proteomic Analysis of Cerebrospinal Fluid in a Fulminant Case of Multiple Sclerosis
Artikel i vetenskaplig tidskrift, 2012

Multiple Sclerosis (MS) is a chronic disease, but in rare fulminant cases rapid progression may lead to death shortly after diagnosis. Currently there is no diagnostic test to predict disease course. The aim of this study was to identify potential biomarkers/proteins related to rapid progression. We present the case history of a 15-year-old male MS patient. Cerebrospinal fluid (CSF) was taken at diagnosis and at the time of rapid progression leading to the patient's death. Using isobaric tag labeling and nanoflow liquid chromatography in conjunction with matrix assisted laser desorption/ionization time of flight tandem mass spectrometry we quantitatively analyzed the protein content of two CSF samples from the patient with fulminant MS as well as one relapsing-remitting (RR) MS patient and one control headache patient, whose CSF analysis was normal. Seventy-eight proteins were identified and seven proteins were found to be more abundant in both fulminant MS samples but not in the RR MS sample compared to the control. These proteins are involved in the immune response, blood coagulation, cell proliferation and cell adhesion. In conclusion, in this pilot study we were able to show differences in the CSF proteome of a rapidly progressing MS patient compared to a more typical clinical form of MS and a control subject.

biomarkers

fulminant

protein quantification

identification

patterns

autoimmune-disease

multiple sclerosis

csf

lesions

diagnosis

vitamin-d

protein

resonance mass-spectrometry

shotgun-proteomics

Författare

J. Fuvesi

University of Szeged

Uppsala universitet

Jörg Hanrieder

Analytisk kemi

Uppsala universitet

K. Bencsik

University of Szeged

C. Rajda

University of Szeged

Filadelfia epilepsihospital

S. K. Kovacs

University of Szeged

L. Kaizer

University of Szeged

S. Beniczky

Filadelfia epilepsihospital

University of Szeged

L. Vecsei

University of Szeged

J. Bergquist

Uppsala universitet

International Journal of Molecular Sciences

1661-6596 (ISSN)

Vol. 13 6 7676-7693

Ämneskategorier

Analytisk kemi

DOI

10.3390/ijms13067676

Mer information

Senast uppdaterat

2022-02-16