Self-assembly formation of multiple DNA-tethered lipid bilayers
Artikel i vetenskaplig tidskrift, 2009

Inspired by natural cell-cell junctions, where membrane-residing proteins control the separation between two or more membranes without interfering with their integrity, we report a new self-assembly route for formation of multiple highly fluid tethered lipid bilayers with the inter-membrane volume geometrically confined by membrane-anchored DNA duplexes. The formation of multiple planar membrane-membrane junctions were accomplished using disk shaped bicelles, composed of a mixture of the long-chained dimyristoyl phosphatidylcholine (DMPC) and the short-chained dihexanoyl PC further stabilized with the positively charged detergent hexadecyl-trimethyl-ammonium bromide (CTAB). Quartz crystal microbalance with dissipation (QCM-D) monitoring and fluorescence microscopy and fluorescence recovery after photobleaching (FRAP) were used to monitor the formation and to characterize the integrity of the self-assembled lipid-DNA architecture. (C) 2009 Elsevier Inc. All rights reserved.

Författare

Seyed Tabaei

Chalmers, Teknisk fysik, Biologisk fysik

Peter Jönsson

Chalmers, Teknisk fysik, Biologisk fysik

Magnus Brändén

Chalmers, Teknisk fysik, Biologisk fysik

Fredrik Höök

Chalmers, Teknisk fysik, Biologisk fysik

Journal of Structural Biology

1047-8477 (ISSN) 1095-8657 (eISSN)

Vol. 168 1 200-206

Styrkeområden

Nanovetenskap och nanoteknik

Livsvetenskaper och teknik

Ämneskategorier

Annan teknik

DOI

10.1016/j.jsb.2009.07.008

Mer information

Skapat

2017-10-06