Glutathione serves an extracellular defence function to decrease arsenite accumulation and toxicity in yeast.
Artikel i vetenskaplig tidskrift, 2012
Arsenic is an environmental toxin and a worldwide health hazard. Since this metalloid is ubiquitous in nature, virtually all living organisms require systems for detoxification and tolerance acquisition. Here, we show that during chronic exposure to arsenite [As(III)], Saccharomyces cerevisiae (budding yeast) exports and accumulates the low-molecular-weight thiol molecule glutathione (GSH) outside of cells. Extracellular accumulation of the arsenite triglutathione complex As(GS)₃ was also detected and direct transport assays demonstrate that As(GS)₃ does not readily enter cells. Yeast cells with increased extracellular GSH levels accumulate less arsenic and display improved growth when challenged with As(III). Conversely, cells defective in export and extracellular accumulation of GSH are As(III) sensitive. Taken together, our data are consistent with a novel detoxification mechanism in which GSH is exported to protect yeast cells from arsenite toxicity by preventing its uptake.
drug effects
metabolism
growth & development
antagonists & inhibitors
Saccharomyces cerevisiae
Drug
antagonists & inhibitors
Arsenites
Antifungal Agents
Metabolic Detoxication
metabolism
metabolism
metabolism
Glutathione
Författare
Michael Thorsen
Göteborgs universitet
Therese Jacobson
Göteborgs universitet
Riet Vooijs
Clara Navarrete Roman
Göteborgs universitet
Tijs Bliek
Henk Schat
Markus J. Tamás
Göteborgs universitet
Molecular Microbiology
0950-382X (ISSN) 1365-2958 (eISSN)
Vol. 84 6 1177-88Ämneskategorier
Biokemi och molekylärbiologi
Mikrobiologi
DOI
10.1111/j.1365-2958.2012.08085.x
PubMed
22554109