Glutathione serves an extracellular defence function to decrease arsenite accumulation and toxicity in yeast.
Artikel i vetenskaplig tidskrift, 2012

Arsenic is an environmental toxin and a worldwide health hazard. Since this metalloid is ubiquitous in nature, virtually all living organisms require systems for detoxification and tolerance acquisition. Here, we show that during chronic exposure to arsenite [As(III)], Saccharomyces cerevisiae (budding yeast) exports and accumulates the low-molecular-weight thiol molecule glutathione (GSH) outside of cells. Extracellular accumulation of the arsenite triglutathione complex As(GS)₃ was also detected and direct transport assays demonstrate that As(GS)₃ does not readily enter cells. Yeast cells with increased extracellular GSH levels accumulate less arsenic and display improved growth when challenged with As(III). Conversely, cells defective in export and extracellular accumulation of GSH are As(III) sensitive. Taken together, our data are consistent with a novel detoxification mechanism in which GSH is exported to protect yeast cells from arsenite toxicity by preventing its uptake.

drug effects

metabolism

growth & development

antagonists & inhibitors

Saccharomyces cerevisiae

Drug

antagonists & inhibitors

Arsenites

Antifungal Agents

Metabolic Detoxication

metabolism

metabolism

metabolism

Glutathione

Författare

Michael Thorsen

Göteborgs universitet

Therese Jacobson

Göteborgs universitet

Riet Vooijs

Clara Navarrete Roman

Göteborgs universitet

Tijs Bliek

Henk Schat

Markus J. Tamás

Göteborgs universitet

Molecular Microbiology

0950-382X (ISSN) 1365-2958 (eISSN)

Vol. 84 6 1177-88

Ämneskategorier

Biokemi och molekylärbiologi

Mikrobiologi

DOI

10.1111/j.1365-2958.2012.08085.x

PubMed

22554109