The IncP-1 plasmid backbone adapts to different host bacterial species and evolves through homologous recombination.
Artikel i vetenskaplig tidskrift, 2011

Plasmids are important members of the bacterial mobile gene pool, and are among the most important contributors to horizontal gene transfer between bacteria. They typically harbour a wide spectrum of host beneficial traits, such as antibiotic resistance, inserted into their backbones. Although these inserted elements have drawn considerable interest, evolutionary information about the plasmid backbones, which encode plasmid related traits, is sparse. Here we analyse 25 complete backbone genomes from the broad-host-range IncP-1 plasmid family. Phylogenetic analysis reveals seven clades, in which two plasmids that we isolated from a marine biofilm represent a novel clade. We also found that homologous recombination is a prominent feature of the plasmid backbone evolution. Analysis of genomic signatures indicates that the plasmids have adapted to different host bacterial species. Globally circulating IncP-1 plasmids hence contain mosaic structures of segments derived from several parental plasmids that have evolved in, and adapted to, different, phylogenetically very distant host bacterial species.

Plasmids

classification

Molecular Sequence Data

genetics

DNA Transposable Elements

Recombination

genetics

Bacteria

Molecular

Evolution

Genetic

Phylogeny

Författare

Peter Norberg

Göteborgs universitet

Maria Bergström

Göteborgs universitet

Vinay Jethava

Chalmers, Data- och informationsteknik, Datavetenskap

Devdatt Dubhashi

Chalmers, Data- och informationsteknik, Datavetenskap

Malte Hermansson

Göteborgs universitet

Nature Communications

2041-1723 (ISSN)

Vol. 2 1 268-

Ämneskategorier

Mikrobiologi

DOI

10.1038/ncomms1267

PubMed

21468020