Towards Classification and Functional Description of Enzymes: A case study of feruloyl esterases
Doktorsavhandling, 2013

The prediction of enzyme functionality from sequence or structure data remains a challenging task that can be best addressed by studying the structure-function relationships determined from previously available information. This thesis work was focused on developing a reliable classification and functional description for the feruloyl esterase (FAE) enzyme family, whose members’ possess both structural and catalytic promiscuity. To establish functional subgrouping of feruloyl esterases a combination of computational and experimental resources was used. The major challenge for FAEs, which often share little sequence similarity to each other and show varied substrate specificity catalyzing the conserved reaction involving an ester bond, is to represent the function in a computationally accessible format. For the analysis of FAEs with overlapping and unique specificity to individual substrates there is a need to capture the chemical function in terms of overall substrate specificity. To meet this requirements, the classification of FAEs was performed by incorporating the information of sequence properties, common-feature based pharmacophore models and the knowledge of active-residue constellations of the FAE binding pockets. Using machine learning techniques an automated descriptor-based classification system for FAEs was proposed that resulted into 12 FAE families. Based on catalytic residue constellations these families were sub-grouped into 32 functionally distinct sub-families. The biological relevance of the descriptor based classification system was validated with experimental data obtained from biochemical and biophysical characterization of FAEs. Challenges in the selection of the appropriate docking algorithm and scoring function combination for the prediction of substrate specificity of FAEs were addressed using molecular docking approaches. The evaluation of 88 docking algorithm-scoring function combinations from leading commercial docking programs for substrate specificity predictions revealed large differences in their performances that could be attributed to the differences in properties of the target proteins. Using the combination of in silico approaches and enzymology, structure-function relationships of FAEs were probed, especially in case of an exceptional Multiple Nucleophilic Elbowed Esterase (MNEE) from Sorangium cellulosum with four functionally distinct and catalytically promiscuous active-sites. Finally, this thesis demonstrates the application of structure-function relationship studies to obtain insights on the promiscuity of enzymes in their evolutionary path and to explain their structure-activity changes in immobilization based biosynthetic reactions.

descriptors

enzyme immobilization

catalytic triad

pharmacophore

enzyme promiscuity

functional classification

molecular docking

feruloyl esterases

protein evolution

structure-function relationship

Lecture Hall HA2, Hörsalsvägen 4, Campus Johanneberg, Chalmers University of Technology, Gothenburg
Opponent: Professor Leif Eriksson

Författare

Gupta Udatha

Chalmers, Kemi- och bioteknik, Industriell Bioteknik

The problem of assigning a biochemical function to newly discovered proteins from the exponential growth of sequencing data has been approached with sequence or structural similarity analysis by researchers. A functional annotation for enzymes usually constitutes an assignment of a class within the Enzyme Commission’s classification system. New functions for existing enzymes are being discovered at a rapid pace and work on revising classification of enzymes has become a continuing operation. Prominent classification approaches that use protein sequences and structures for classification of enzymes have been considered inefficient, since enzymes belonging to the same class are neither necessarily similar in sequence/structure nor the enzymes that are similar in sequence/structure are necessarily similar in function. The objective of my thesis work was to understand the structural and functional promiscuity of enzymes and to provide an efficient scheme towards their functional classification, by taking feruloyl esterases as a case study. By applying bioinformatics, cheminformatics and protein biochemistry tools in enzymology, I aimed at (a) developing a reliable classification scheme for feruloyl esterases that can overcome the challenges posed by their promiscuous properties for functional sub-grouping, and (b) to understand the structure-function relationships of feruloyl esterases for their use in biosynthetic applications.

Ämneskategorier

Industriell bioteknik

Biokemi och molekylärbiologi

Biofysik

Bioinformatik och systembiologi

Biokatalys och enzymteknik

Styrkeområden

Livsvetenskaper och teknik

ISBN

978-91-7385-770-3

Doktorsavhandlingar vid Chalmers tekniska högskola. Ny serie: 3451

Lecture Hall HA2, Hörsalsvägen 4, Campus Johanneberg, Chalmers University of Technology, Gothenburg

Opponent: Professor Leif Eriksson

Mer information

Skapat

2017-10-07