Gelatin methacrylate as a promising hydrogel for 3D microscale organization and proliferation of dielectrophoretically patterned cells
Artikel i vetenskaplig tidskrift, 2012

Establishing the 3D microscale organization of cells has numerous practical applications, such as in determining cell fate (e. g., proliferation, migration, differentiation, and apoptosis) and in making functional tissue constructs. One approach to spatially pattern cells is by dielectrophoresis (DEP). DEP has characteristics that are important for cell manipulation, such as high accuracy, speed, scalability, and the ability to handle both adherent and non-adherent cells. However, widespread application of this method is largely restricted because there is a limited number of suitable hydrogels for cell encapsulation. To date, polyethylene glycol-diacrylate (PEG-DA) and agarose have been used extensively for dielectric patterning of cells. In this study, we propose gelatin methacrylate (GelMA) as a promising hydrogel for use in cell dielectropatterning because of its biocompatibility and low viscosity. Compared to PEG hydrogels, GelMA hydrogels showed superior performance when making cell patterns for myoblast (C2C12) and endothelial (HUVEC) cells as well as in maintaining cell viability and growth. We also developed a simple and robust protocol for co-culture of these cells. Combined application of the GelMA hydrogels and the DEP technique is suitable for creating highly complex microscale tissues with important applications in fundamental cell biology and regenerative medicine in a rapid, accurate, and scalable manner.

Författare

J. Ramon-Azcon

Tohoku University

S. Ahadian

Tohoku University

R. Obregon

Tohoku University

G. Camci-Unal

Massachusetts Institute of Technology (MIT)

Brigham and Women's Hospital

S. Ostrovidov

Tohoku University

Vahid Hosseini

Chalmers, Kemi- och bioteknik

H. Kaji

Tohoku University

K. Ino

Tohoku University

H. Shiku

Tohoku University

A. Khademhosseini

Harvard University

Kyung Hee University

Tohoku University

Brigham and Women's Hospital

Massachusetts Institute of Technology (MIT)

T. Matsue

Tohoku University

Lab on a Chip - Miniaturisation for Chemistry and Biology

1473-0197 (ISSN) 1473-0189 (eISSN)

Vol. 12 2959-2969

Ämneskategorier

Biologiska vetenskaper

DOI

10.1039/c2lc40213k