Serum from patients with chronic idiopathic thrombocytopenic purpura frequently affect the platelet function.
Artikel i vetenskaplig tidskrift, 2002

In most, but not all, cases of chronic idiopathic thrombocytopenic purpura (ITP), bleeding complications are known to occur when the platelet count is low. The present study investigates the effect of ITP sera on the in vitro platelet function of donor platelets. Sera from 58 ITP patients were investigated. Using an indirect monoclonal antibody specific immobilisation of platelet antigen (MAIPA) technique, GPIIb/IIIa and GPIb/IX specific antibodies were found in 23 and 20 patients, respectively. Twelve of them had antibodies against both glycoprotein (GP) complexes. The ITP sera's effect on donor platelets was investigated by aggregometry and the results were compared with the ones of 26 healthy donor sera. Grouped together, the ITP sera significantly impaired the ADP-induced platelet aggregation of donor platelets compared to the control sera; the mean relative aggregation response (T(max)) seen for the ITP and control sera were 82 +/- 21% and 92 +/- 7%, respectively (p = 0.0157). However, 6 ITP sera gave an enhanced aggregation response, whereas 17 ITP sera resulted in an impaired platelet aggregation, when using the mean +/- 2 S.D. recorded for the controls as the normal range. There was not any correlation between aggregation response, platelet number or the presence of GPIb/IX or GPIIb/IIIa specific antibodies, other than the fact that all ITP sera causing an enhanced aggregation were from patients with a platelet number less then 100 x 10(9)/l at the time of blood sampling. It is concluded that some ITP sera can either enhance or impair the platelet aggregation response, but in most cases, a normal response is obtained.

80 and over

Aged

Adult

Adenosine Diphosphate

Middle Aged

immunology

Platelet Glycoprotein GPIIb-IIIa Complex

Adolescent

Platelet Glycoprotein GPIb-IX Complex

Platelet Membrane Glycoproteins

Aged

immunology

blood

Platelet Aggregation

drug effects

Autoantibodies

Kinetics

Platelet Count

immunology

pharmacology

Thrombocytopenic

Female

blood

Case-Control Studies

Purpura

Male

Idiopathic

Humans

Författare

Per-Ola Andersson

Göteborgs universitet

Lilian Tengborn

Hans Wadenvik

Göteborgs universitet

Thrombosis Research

0049-3848 (ISSN)

Vol. 107 3-4 135-9

Ämneskategorier

Hematologi

PubMed

12431479

Mer information

Skapat

2017-10-10