The Multifunctional Pipette. A Microfluidic Technology for the Biosciences
Doktorsavhandling, 2013

The theme of the work described in this thesis is the generation and application of liquid microenvironments in chemistry and bioscience using microfluidic devices. First, a computer controlled multi-stage dilution system to generate time-dependent chemical waves was developed, and its application was demonstrated on model biomembranes. Thereafter the focus was shifted towards spatial control of chemistry. Using a hydrodynamic flow confinement concept in an open liquid volume, we created a device coined “Multifunctional Pipette”. It features localized liquid handling at the single-cell size scale together with fast solution exchange. The technology has been refined and optimized to provide a feature-rich tool for biologists working with cells and tissues in microscopy experiments. Application examples include cell zeiosis, single-cell dose-response determination and ion-channel stimulation. Subsequent studies cover modifications and applications of this device, such as on-chip electrodes and electroporation, as well as uses in cell cultures, on tissue slices, and as an optofluidic thermometer. Finally, localized liquid handling has been applied to assemble 2-dimensional fluidic networks consisting of directly written supported lipid bilayers. This "Lab on a Membrane" toolbox allows rapid prototyping of 2D-fluidic circuits, to modify their chemistry and connectivity on-demand and to apply them in studies of molecular interactions.

microfluidic superfusion

microfluidic temperature sensing.

microfluidic dilution

hydrodynamic flow confinement

microfluidic mixer

supported lipid membranes

single-cell analysis

Microfluidics

microfabrication

PDMS

sal KA, Kemigården 4, Chalmers, Göteborg
Opponent: Prof. George M. Whitesides

Författare

Alar Ainla

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

A Microfluidic Diluter Based on Pulse Width Flow Modulation

Analytical Chemistry,; Vol. 81(2009)p. 5549-5556

Artikel i vetenskaplig tidskrift

A multifunctional pipette

Lab on a Chip - Miniaturisation for Chemistry and Biology,; Vol. 12(2012)p. 1255-1261

Artikel i vetenskaplig tidskrift

A Microfluidic Pipette for Single-Cell Pharmacology

Analytical Chemistry,; Vol. 82(2010)p. 4529-4536

Artikel i vetenskaplig tidskrift

Single-cell electroporation using a multifunctional pipette

Lab on a Chip - Miniaturisation for Chemistry and Biology,; Vol. 12(2012)p. 4605-4609

Artikel i vetenskaplig tidskrift

Styrkeområden

Nanovetenskap och nanoteknik

Ämneskategorier

Fysikalisk kemi

Analytisk kemi

Nanoteknik

Infrastruktur

Nanotekniklaboratoriet

ISBN

978-91-7385-818-2

Doktorsavhandlingar vid Chalmers tekniska högskola. Ny serie

sal KA, Kemigården 4, Chalmers, Göteborg

Opponent: Prof. George M. Whitesides

Mer information

Skapat

2017-10-07