Repair of large area pores in supported double bilayers
Artikel i vetenskaplig tidskrift, 2013

We describe an experimental system where we can generate, and subsequently close, multiple large membrane ruptures in supported double bilayers. We show in this study for the first time that large membrane pores (similar to 10-150 mu m in size) in flat phospholipid vesicles can be reduced in size or completely closed by a pore edge tension driven area reduction mechanism. We can dynamically control the membrane tension of a flat giant unilamellar vesicle and its interplay with the surface adhesion to a solid support. Adhesion to the support surface causes increased membrane tension, which eventually relaxes by the formation of several pores in the membrane. We show that the tension propagation time tau(max) is exceptionally long in this system, which allows for simultaneous opening of multiple pores. The pores can be stabilized by Ca2+-mediated pinning sites in the interior of the flat giant unilamellar vesicle. After pore formation followed by pinning, we depleted Ca2+ ions resulting in removal of pinning and relaxation of membrane tension. This allows the pore to close, driven by the pore edge tension.

binding

phase

plasma-membrane

vesicles

phospholipid-membranes

spectroscopy

cells

calcium

Författare

Irep Gözen

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

B. Ortmen

Chalmers University of Technology

I. Poldsalu

Tartu Ülikool

P. Dommersnes

Centre for Advanced Study

Owe Orwar

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Aldo Jesorka

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Soft Matter

1744-683X (ISSN) 1744-6848 (eISSN)

Vol. 9 10 2787-2792

Ämneskategorier

Biologiska vetenskaper

Kemi

DOI

10.1039/c3sm27429b