Recellularization of acellular human small intestine using bone marrow stem cells.
Artikel i vetenskaplig tidskrift, 2013

We aimed to produce an acellular human tissue scaffold with a view to test the possibility of recellularization with bone marrow stem cells to produce a tissue-engineered small intestine (TESI). Human small-bowel specimens (n = 5) were obtained from cadaveric organ donors and treated sequentially with 6% dimethyl sulfoxide in hypotonic buffer, 1% Triton X-100, and DNase. Each small intestine (SI) piece (6 cm) was recellularized with EPCAM+ and CD133+ allogeneic bone marrow stem cells. Histological and molecular analysis demonstrated that after decellularization, all cellular components and nuclear material were removed. Our analysis also showed that the decellularized human SI tissue retained its histoarchitecture with intact villi and major structural proteins. Protein films of common extracellular matrix constituents (collagen I, laminin, and fibronectin) were found in abundance. Furthermore, several residual angiogenic factors were found in the decellularized SI. Following recellularization, we found viable mucin-positive goblet cells, CK18+ epithelial cells in villi adjacent to a muscularis mucosa with α-actin+ smooth muscle cells, and a high repopulation of blood vessels with CD31+ endothelial cells. Our results show that in the future, such a TESI would be ideal for clinical purposes, because it can be derived from the recipient's own immunocompatible bone marrow cells, thus avoiding the use of immunosuppression.

Adult stem cells

Adult human bone marrow

Differentiation

Transdifferentiation

Författare

Pradeep B Patil

Göteborgs universitet

Priti Chougule

Göteborgs universitet

Vijay Kumar Kuna

Göteborgs universitet

Stefan Almström

Henrik Bäckdahl

Debashish Banerjee

Göteborgs universitet

Gustaf Herlenius

Michael Olausson

Göteborgs universitet

Suchitra Sumitran-Holgersson

Göteborgs universitet

Stem cells translational medicine

2157-6564 (ISSN) 2157-6580 (eISSN)

Vol. 2 307-15

Ämneskategorier

Kirurgi

DOI

10.5966/sctm.2012-0108

PubMed

23486834