Measuring absolute nanoparticle number concentrations from particle count time series
Artikel i vetenskaplig tidskrift, 2013

Single-particle microscopy is important for characterization of nanoparticulate matter for which accurate concentration measurements are crucial. We introduce a method for estimating absolute number concentrations in nanoparticle dispersions based on a fluctuating time series of particle counts, known as a Smoluchowski process. Thus, unambiguous tracking of particles is not required and identification of single particles is sufficient. However, the diffusion coefficient of the particles must be estimated separately. The proposed method does not require precalibration of the detection region volume, as this can be estimated directly from the observations. We evaluate the method in a simulation study and on experimental data from a series of dilutions of 0.2- and 0.5-m polymer nanospheres in water, obtaining very good agreement with reference values.

single-particle methods

size

Concentration measurements

Smoluchowski

fluorescence microscopy

fluctuations

physics

interface

delivery

Författare

Magnus Röding

SuMo Biomaterials

Chalmers, Matematiska vetenskaper, Matematisk statistik

Göteborgs universitet

Hendrik Deschout

Universiteit Gent

Kevin Braeckmans

Universiteit Gent

Mats Rudemo

Göteborgs universitet

Chalmers, Matematiska vetenskaper, Matematisk statistik

Journal of Microscopy

0022-2720 (ISSN) 1365-2818 (eISSN)

Vol. 251 1 19-26

Ämneskategorier

Matematik

DOI

10.1111/jmi.12038

Mer information

Senast uppdaterat

2020-08-18