High incidence of DNA mutations and gene amplifications of the ALK gene in advanced sporadic neuroblastoma tumours.
Artikel i vetenskaplig tidskrift, 2008

ALK (anaplastic lymphoma kinase) is oncogenic in several tumours and has recently been identified as a predisposition gene for familial NB (neuroblastoma) harbouring mutations in the TKD (tyrosine kinase domain). We have analysed a large set of sporadic human NB primary tumours of all clinical stages for chromosomal re-arrangements using a CGH (comparative genomic hybridization) array (n=108) and mutations of the ALK gene (n=90), and expression of ALK and related genes (n=19). ALK amplification or in-gene re-arrangements were found in 5% of NB tumours and mutations were found in 11%, including two novel not previously published mutations in the TKD, c.3733T>A and c.3735C>A. DNA mutations in the TKD and gene amplifications were only found in advanced large primary tumours or metastatic tumours, and correlated with the expression levels of ALK and downstream genes as well as other unfavourable features, and poor outcome. The results of the present study support that the ALK protein contributes to NB oncogenesis providing a highly interesting putative therapeutic target in a subset of unfavourable NB tumours.

Mutation

genetics

Gene Amplification

genetics

Neuroblastoma

DNA Primers

Single Nucleotide

mortality

Polymorphism

genetics

pathology

Polymerase Chain Reaction

Humans

Oligonucleotide Array Sequence Analysis

Neoplasm Staging

DNA

Survival Analysis

Protein-Tyrosine Kinases

Receptor Protein-Tyrosine Kinases

Neoplasm

Författare

Helena Carén

Göteborgs universitet

Frida Abel

Göteborgs universitet

Per Kogner

Tommy Martinsson

Göteborgs universitet

Biochemical Journal

0264-6021 (ISSN) 1470-8728 (eISSN)

Vol. 416 153-9

Ämneskategorier

Klinisk medicin

PubMed

18990089