Continuous Cultivation of Dilute-Acid Hydrolysates to Ethanol by Immobilized Saccharomyces cerevisiae
Artikel i vetenskaplig tidskrift, 2001

The continuous cultivation of immobilized Saccharomyces cerevisiae CBS 8066 on dilute-acid hydrolysates of forest residuals was investigated. The yeast cells were immobilized in 2–4% Ca-alginate beads. The 2% beads were not stable. However, the 3 and 4% beads were stable for at least 3 wk when an extra resource of calcium ions was available in the medium. The continuous cultivation of a dilute-acid hydrolysate by the immobilized cells at dilution rates of 0.3, 0.5, and 0.6 h−1 resulted in 86, 83, and 79% sugar consumption, respectively, and an ethanol yield between 0.45 and 0.48 g/g. The hydrolysate was fermentable at a dilution rate of 0.1 h−1 in a free-cell system but washed out at a dilution rate of 0.2 h−1. The continuous cultivation of a more inhibiting hydrolysate was not successful by either free- or immobilized-cell systems even at a low dilution rate of 0.07 h−1. However, when the hydrolysate was overlimed, it was fermentable by the immobilized cells at a dilution rate of 0.2 h−1.

glucose

Saccharomyces cerevisiae

immobilization.

dilute–acid hydrolysate

ethanol

Författare

Mohammad Taherzadeh Esfahani

Institutionen för kemisk reaktionsteknik

Claes Niklasson

Institutionen för kemisk reaktionsteknik

Ria Millati

Institutionen för kemisk reaktionsteknik

Applied Biochemistry and Biotechnology

0273-2289 (ISSN)

Vol. 95 45-57

Ämneskategorier

Bioprocessteknik

Styrkeområden

Livsvetenskaper och teknik