Real-time monitoring of surface-confined platelet activation on TiO2
Artikel i vetenskaplig tidskrift, 2014

For the development of advanced hemocompatible biomaterial functions, there is an unmet demand for in vitro evaluation techniques addressing platelet-surface interactions. We show that the quartz crystal microbalance with dissipation (QCM-D) monitoring technique, here combined with light microscopy, provides a surface sensitive technique that allows for real-time monitoring of the activation and aggregation of the surface-confined platelets on TiO2. The QCM-D signal monitored during adhesion and activation of platelets on TiO2 coated surfaces was found to be different in platelet-poor and platelet-rich environment although light microscopy images taken for each of the two cases looked essentially the same. Interestingly, aggregation of activated platelets was only observed in a protein-rich environment. Our results show that a layer of plasma proteins between the TiO2 surface and the platelets strongly influences the coupling between the platelets and the underlying substrate, explaining both the observed QCM-D signals and the ability of the platelets to aggregate. © 2014 The Authors.

Platelet activation

QCM-D

Aggregation

TiO2

Författare

Angelika Kunze

Chalmers, Teknisk fysik, Biologisk fysik

Camilla Hesse

Göteborgs universitet

Sofia Svedhem

Chalmers, Teknisk fysik, Biologisk fysik

Colloids and Surfaces B: Biointerfaces

0927-7765 (ISSN)

Vol. 116 446-451

Ämneskategorier

Den kondenserade materiens fysik

DOI

10.1016/j.colsurfb.2014.01.025

PubMed

24549046