In Vitro Models for Studying Secondary Plant Metabolite Digestion and Bioaccessibility
Artikel i vetenskaplig tidskrift, 2014

There is an increased interest in secondary plant metabolites, such as polyphenols and carotenoids, due to their proposed health benefits. Much attention has focused on their bioavailability, a prerequisite for further physiological functions. As human studies are time consuming, costly, and restricted by ethical concerns, in vitro models for investigating the effects of digestion on these compounds have been developed and employed to predict their release from the food matrix, bioaccessibility, and assess changes in their profiles prior to absorption. Most typically, models simulate digestion in the oral cavity, the stomach, the small intestine, and, occasionally, the large intestine. A plethora of models have been reported, the choice mostly driven by the type of phytochemical studied, whether the purpose is screening or studying under close physiological conditions, and the availability of the model systems. Unfortunately, the diversity of model conditions has hampered the ability to compare results across different studies. For example, there is substantial variability in the time of digestion, concentrations of salts, enzymes, and bile acids used, pH, the inclusion of various digestion stages; and whether chosen conditions are static (with fixed concentrations of enzymes, bile salts, digesta, and so on) or dynamic (varying concentrations of these constituents). This review presents an overview of models that have been employed to study the digestion of both lipophilic and hydrophilic phytochemicals, comparing digestive conditions in vitro and in vivo and, finally, suggests a set of parameters for static models that resemble physiological conditions.

in vitro models

DYNAMIC GUT MODEL

V34

GREEN TEA CATECHINS

P197

BAQCHA.S

HUMAN INTESTINAL MICROBIOTA

WINE/GRAPE JUICE

ATLA-ALTERNATIVES TO LABORATORY ANIMALS

SIMULATED GASTROINTESTINAL DIGESTION

bioacessibility

CACO-2 CELLS

BETA-CAROTENE

LLER DD

P2248

gastrointestinal digestion

CELL-CULTURE MODEL

1995

polyphenols

phytochemicals

1968

AMERICAN JOURNAL OF CLINICAL NUTRITION

GASTRIC-ACID SECRETION

NEKUS M

Food Science & Technology

SWEET-POTATO

V2

LANCET

FLESHED

P12

RED

V23

1981

carotenoids

Författare

Marie Alminger

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Livsmedelsvetenskap

A. M. Aura

T. Bohn

C. Dufour

S. N. El

A. Gomes

S. Karakaya

M. C. Martinez-Cuesta

G. J. McDougall

T. Requena

C. N. Santos

Gastroenterology V. P. Rriere F

British Journal Of Nutrition V. P. Rry Jl

Comprehensive Reviews in Food Science and Food Safety

1541-4337 (ISSN)

Vol. 13 413-436

Ämneskategorier

Livsmedelsvetenskap

Livsmedelsteknik

Styrkeområden

Livsvetenskaper och teknik

DOI

10.1111/1541-4337.12081