Intermethod comparison of the particle size distributions of colloidal silica nanoparticles
Artikel i vetenskaplig tidskrift, 2014

There can be a large variation in the measured diameter of nanoparticles depending on which method is used. In this work, we have strived to accurately determine the mean particle diameter of 3040 nm colloidal silica particles by using six different techniques. A quantitative agreement between the particle size distributions was obtained by scanning electron microscopy (SEM), and electrospray-scanning mobility particle sizer (ES SMPS). However, transmission electron microscopy gave a distribution shifted to smaller sizes. After confirming that the magnification calibration was consistent, this was attributed to sample preparation artifacts. The hydrodynamic diameter, d(h), was determined by dynamic light scattering (DLS) both in batch mode, and hyphenated with sedimentation field flow fractionation. Surprisingly the dh were smaller than the SEM, and ES SMPS diameters. A plausible explanation for the smaller sizes found with DLS is that a permeable gel layer forms on the particle surface. Results from nanoparticle tracking analysis were strongly biased towards larger diameters, most likely because the silica particles provide low refractive index contrast. Calculations confirmed that the sensitivity is, depending on the shape of the laser beam, strongly size dependent for particles with diameters close to the visualization limit.

particle morphology

method comparison

validation

particle diameter

surface properties

gel layer

Författare

Jani Tuoriniemi

Göteborgs universitet

Ann-Catrin J. H. Johnsson

Göteborgs universitet

Jenny Perez-Holmberg

Göteborgs universitet

Stefan Gustafsson

SuMo Biomaterials

Chalmers, Teknisk fysik, Eva Olsson Group

Julian A. Gallego-Urrea

Göteborgs universitet

Eva Olsson

Chalmers, Teknisk fysik, Eva Olsson Group

Jan B. C. Pettersson

Göteborgs universitet

Martin Hassellöv

Göteborgs universitet

Science and Technology of Advanced Materials

1468-6996 (ISSN)

Vol. 15 3 artikel 035009- 035009

Ämneskategorier

Analytisk kemi

DOI

10.1088/1468-6996/15/3/035009

Mer information

Skapat

2018-01-24