Clustered DNA damage on subcellular level: effect of scavengers
Artikel i vetenskaplig tidskrift, 2014

Clustered DNA damages are induced by ionizing radiation, particularly of high linear energy transfer (LET). Compared to isolated DNA damage sites, their biological effects can be more severe. We investigated a clustered DNA damage induced by high LET radiation (C 290 MeV u(-1) and Fe 500 MeV u(-1)) in pBR322 plasmid DNA. The plasmid is dissolved in pure water or in aqueous solution of one of the three scavengers (coumarin-3-carboxylic acid, dimethylsulfoxide, and glycylglycine). The yield of double strand breaks (DSB) induced in the DNA plasmid-scavenger system by heavy ion radiation was found to decrease with increasing scavenging capacity due to reaction with hydroxyl radical, linearly with high correlation coefficients. The yield of non-DSB clusters was found to occur twice as much as the DSB. Their decrease with increasing scavenging capacity had lower linear correlation coefficients. This indicates that the yield of non-DSB clusters depends on more factors, which are likely connected to the chemical properties of individual scavengers.

Heavy ion

Plasmid in liquid water

Scavenger

Indirect effects

Clustered damage

Författare

Katerina Pachnerova Brabcová

Chalmers, Teknisk fysik, Nukleär teknik

Lembit Sihver

Chalmers, Teknisk fysik, Nukleär teknik

N. Yasuda

University of Fukui

Y. Matuo

University of Fukui

V. Stepan

Centre d'Etudes Nucleaires de Bordeaux Gradignan

Czech Academy of Sciences

M. Davidkova

Czech Academy of Sciences

Radiation and Environmental Biophysics

0301-634X (ISSN) 1432-2099 (eISSN)

Vol. 53 705-712

Ämneskategorier

Annan teknik

DOI

10.1007/s00411-014-0557-2