Genetic associations of Nrf2-encoding NFE2L2 variants with Parkinson's disease: a multicenter study
Artikel i vetenskaplig tidskrift, 2014

Background: The transcription factor Nrf2, encoded by the NFE2L2 gene, is an important regulator of the cellular protection against oxidative stress. Parkinson s disease is a neurodegenerative disease highly associated with oxidative stress. In a previously published study, we reported associations of NFE2L2 haplotypes with risk and age at onset of idiopathic Parkinson s disease in a Swedish discovery material and a Polish replication material. Here, we have extended the replication study and performed meta-analyses including the Polish material and four new independent European patient-control materials. Furthermore, all SNPs included in the haplotype windows were investigated individually for associations with Parkinson s disease in meta-analyses including all six materials. Methods: Totally 1038 patients and 1600 control subjects were studied. Based on previous NFE2L2 haplotype associations with Parkinson s disease, five NFE2L2 tag SNPs were genotyped by allelic discrimination and three functional NFE2L2 promoter SNPs were genotyped by sequencing. The impact of individual SNPs and haplotypes on risk and age at onset of Parkinson s disease were investigated in each material individually and in meta-analyses of the obtained results. Results: Meta-analyses of NFE2L2 haplotypes showed association of haplotype GAGCAAAA, including the fully functional promoter haplotype AGC, with decreased risk (OR = 0.8 per allele, p = 0.012) and delayed onset (+ 1.1 years per allele, p = 0.048) of Parkinson s disease. These results support the previously observed protective effect of this haplotype in the first study. Further, meta-analyses of the SNPs included in the haplotypes revealed four NFE2L2 SNPs associated with age at onset of Parkinson s disease (rs7557529 G > A, -1.0 years per allele, p = 0.042; rs35652124 A > G, -1.1 years per allele, p = 0.045; rs2886161 A > G, -1.2 years per allele, p = 0.021; rs1806649 G > A, + 1.2 years per allele, p = 0.029). One of these (rs35652124) is a functional SNP located in the NFE2L2 promoter. No individual SNP was associated with risk of Parkinson s disease. Conclusion: Our results support the hypothesis that variation in the NFE2L2 gene, encoding a central protein in the cellular protection against oxidative stress, may contribute to the pathogenesis of Parkinson s disease. Functional studies are now needed to explore these results further.

Parkinson s disease

Risk factor









Malin von Otter

Göteborgs universitet

Petra Bergström

Göteborgs universitet

Aldo Quattrone

Universita degli studi Magna Graecia di Catanzaro

Consiglio Nazionale delle Ricerche

Elvira De Marco

Consiglio Nazionale delle Ricerche

Grazia Annesi

Consiglio Nazionale delle Ricerche

Peter Söderkvist

Linköpings universitet

Stephanie Wettinger

University of Malta

Marek Drozdzik

Pomeranian Medical University in Szczecin

Monika Bialecka

Pomeranian Medical University in Szczecin

Hans Nissbrandt

Göteborgs universitet

Christine Klein

Universitaet Zu Lübeck

Michael Nilsson

Göteborgs universitet

Ola Hammarsten

Göteborgs universitet

Staffan Nilsson

Göteborgs universitet

Chalmers, Matematiska vetenskaper, matematisk statistik

Henrik Zetterberg

Göteborgs universitet

BMC Medical Genetics

1471-2350 (ISSN)

Vol. 15 artikel nr 131- 131




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