Complexation of aluminum with DNA.
Artikel i vetenskaplig tidskrift, 1987

The extent of complexation of aluminum(III) with DNA (Calf thymus, Sigma type I) was estimated by means of two experimental techniques: potentiometric titration with a fluoride selective indicator electrode and dialysis followed by aluminum determination by graphite furnace AAS. Both types of experiments indicate that aluminum(III) is bound to DNA. The data are treated by assuming an ion exchange reaction with the phosphate diester groups. Using Rt to denote the concentration of these groups the values of log [AlMn-3R]/(Rt-3[AlMn-3R])[Al3+] decrease from approx. 7.6 to 5.6 when the concentration of sodium chloride is increased from 1 to 100 mM. In the pH range 4.5-5.5 the ion exchange constant increases approximately 0.5 log units. Dialysis gives lower values for the complex formation constant than potentiometry.

Osmolar Concentration

Spectrophotometry

Cations

Aluminum

Chemical Phenomena

Potentiometry

Chemistry

DNA

Författare

David Dyrssen

Institutionen för analytisk och marin kemi

Göteborgs universitet

Conny Haraldsson

Göteborgs universitet

Institutionen för analytisk och marin kemi

Eva Nyberg

Göteborgs universitet

Institutionen för analytisk och marin kemi

Margareta Wedborg

Göteborgs universitet

Institutionen för analytisk och marin kemi

Journal of Inorganic Biochemistry

0162-0134 (ISSN)

Vol. 29 1 67-75

Ämneskategorier

Analytisk kemi

Kemi

PubMed

3559548