Non-Invasive Acoustical sensing of Drug-Induced Effects on the Contractile Machinery of Human Cardiomyocyte Clusters
Artikel i vetenskaplig tidskrift, 2015

There is an urgent need for improved models for cardiotoxicity testing. Here we propose acoustic sensing applied to beating human cardiomyocyte clusters for non-invasive, surrogate measuring of the QT interval and other characteristics of the contractile machinery. In experiments with the acoustic method quartz crystal microbalance with dissipation monitoring (QCM-D), the shape of the recorded signals was very similar to the extracellular field potential detected in electrochemical experiments, and the expected changes of the QT interval in response to addition of conventional drugs (E-4031 or nifedipine) were observed. Additionally, changes in the dissipation signal upon addition of cytochalasin D were in good agreement with the known, corresponding shortening of the contraction-relaxation time. These findings suggest that QCM-D has great potential as a tool for cardiotoxicological screening, where effects of compounds on the cardiomyocyte contractile machinery can be detected independently of whether the extracellular field potential is altered or not.

Författare

Angelika Kunze

Chalmers, Teknisk fysik, Biologisk fysik

D. Steel

Cellectis AB

Abcam plc

K. Dahlenborg

Cellectis AB

P. Sartipy

Högskolan i Skövde

Cellectis AB

AstraZeneca AB

Sofia Svedhem

Chalmers, Teknisk fysik, Biologisk fysik

PLoS ONE

1932-6203 (ISSN)

Vol. 10 5 Art. no. e0125540- e0125540

Nanopartiklar och deras interaktioner med biologiska gränsytor - medicinska tillämpningar och nanosäkerhet

Vetenskapsrådet (VR), 2013-01-01 -- 2015-12-31.

Ämneskategorier

Annan teknik

Analytisk kemi

DOI

10.1371/journal.pone.0125540

Mer information

Senast uppdaterat

2018-03-21