Bridging omics technologies with synthetic biology in yeast industrial biotechnology
Kapitel i bok, 2013

Metabolic engineering, defined as the practice of manipulating cells' genetic and regulatory processes for improving cellular performance, has been recently integrating systems and synthetic biology with other technologies, e.g. molecular biology, physiology, biochemistry, analysis science, bioinformatics and biochemical engineering. The aim is to surmount cells' performance-related limitations, e.g. metabolic limitation of cellular processes, network rigidity and global regulation, in a comprehensive, rational and high-throughput manner. Using this multi-level/integrated approach, the (re)design and (re)construction of microbial systems for the development of novel products with significant impact on current global problems, e.g. depletion of energy resources and global warming, is becoming a reality. In the last two decades, technological platforms for systems biology, e.g. genomics, transcriptomics, proteomics, metabolomics and fluxomics, and synthetic biology approaches, e.g. synthetic biological parts, devices and systems, have been implemented and efficiently used as tools for metabolic engineering of high-value bioproducts. One successful story on how metabolic engineering has been used to enhance the synthesis of microbial-based molecules is the production of biofuels. In this review, synthetic and systems biology technologies for yeast metabolic engineering will be described in detail. In addition, two case studies related with biofuel production (ethanol and 1-butanol) in yeast S. cerevisiae will be presented.

Systems biology

Biofuels

Synthetic biology

Metabolic engineering

Antisense RNA

Data mining

Industrial biotechnology

Genomics

Butanol

Riboswitches

Promoter binding proteins

Genome-scale metabolic models

Ribozymes

Ethanol

Metabolomics

Transcriptomics

Synthetic pathway

Riboregulators

Promoter libraries

Illumina sequencing

Targeted gene expression

Renewable resources

Proteomics

Promoter shuffling

Multi-gene assembly

High-value bioproducts

Yeast

Protein scaffolds

Microarrays

RNAseq

Fluxomics

Saccharomyces cerevisiae

Författare

Antonio Roldao

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

Il-Kwon Kim

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

Jens B Nielsen

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

Systems Metabolic Engineering

271-327

Ämneskategorier

Biologiska vetenskaper

DOI

10.1007/978-94-007-4534-6_9

ISBN

978-94-007-4533-9