Bridging omics technologies with synthetic biology in yeast industrial biotechnology
Kapitel i bok, 2012

Metabolic engineering, defined as the practice of manipulating cells' genetic and regulatory processes for improving cellular performance, has been recently integrating systems and synthetic biology with other technologies, e.g. molecular biology, physiology, biochemistry, analysis science, bioinformatics and biochemical engineering. The aim is to surmount cells' performance-related limitations, e.g. metabolic limitation of cellular processes, network rigidity and global regulation, in a comprehensive, rational and high-throughput manner. Using this multi-level/integrated approach, the (re)design and (re)construction of microbial systems for the development of novel products with significant impact on current global problems, e.g. depletion of energy resources and global warming, is becoming a reality. In the last two decades, technological platforms for systems biology, e.g. genomics, transcriptomics, proteomics, metabolomics and fluxomics, and synthetic biology approaches, e.g. synthetic biological parts, devices and systems, have been implemented and efficiently used as tools for metabolic engineering of high-value bioproducts. One successful story on how metabolic engineering has been used to enhance the synthesis of microbial-based molecules is the production of biofuels. In this review, synthetic and systems biology technologies for yeast metabolic engineering will be described in detail. In addition, two case studies related with biofuel production (ethanol and 1-butanol) in yeast S. cerevisiae will be presented.

Microarrays

Genomics

Ribozymes

Promoter libraries

Synthetic biology

Butanol

Proteomics

Biofuels

RNAseq

Targeted gene expression

Fluxomics

Riboswitches

Protein scaffolds

Promoter shuffling

Transcriptomics

Illumina sequencing

High-value bioproducts

Riboregulators

Multi-gene assembly

Saccharomyces cerevisiae

Metabolic engineering

Antisense RNA

Genome-scale metabolic models

Yeast

Metabolomics

Industrial biotechnology

Synthetic pathway

Ethanol

Systems biology

Data mining

Renewable resources

Promoter binding proteins

Författare

Antonio Roldao

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

Il-Kwon Kim

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

Jens B Nielsen

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

Systems Metabolic Engineering

271-327

Ämneskategorier

Biologiska vetenskaper

DOI

10.1007/978-94-007-4534-6_9

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Senast uppdaterat

2021-07-26