Micro-droplet based directed evolution outperforms conventional laboratory evolution
Paper i proceeding, 2015

We present droplet adaptive laboratory evolution (DrALE), a directed evolution method used to improve industrial enzyme producing microorganisms for e.g. feedstock digestion. DrALE is based linking a desired phenotype to growth rate allowing only desired cells to proliferate. Single cells are confined in microfluidic droplets to prevent the phenotype, e.g. secreted enzymes, from leaking between cells. The method was benchmarked against and found to significantly outperform conventional adaptive laboratory evolution (ALE) in enriching enzyme producing cells. It was furthermore applied to enrich a whole-genome mutated library of yeast cells for α-amylase activity.

High throughput

Directed evolution

Cell factories

Droplet microfluidics

Enzymes

Författare

S. L. Sjostrom

Kungliga Tekniska Högskolan (KTH)

Mingtao Huang

Chalmers, Biologi och bioteknik, Systembiologi

Jens B Nielsen

Chalmers, Biologi och bioteknik, Systembiologi

H. N. Joensson

Kungliga Tekniska Högskolan (KTH)

H. A. Svahn

Kungliga Tekniska Högskolan (KTH)

18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014; United States; 26-30 October 2014

169-171

Styrkeområden

Livsvetenskaper och teknik

Ämneskategorier

Bioinformatik och systembiologi

ISBN

978-097980647-6