CONTRIBUTION OF INDIRECT EFFECTS TO CLUSTERED DAMAGE IN DNA IRRADIATED WITH PROTONS
Artikel i vetenskaplig tidskrift, 2015

Protons are the dominant particles both in galactic cosmic rays and in solar particle events and, furthermore, proton irradiation becomes increasingly used in tumour treatment. It is believed that complex DNA damage is the determining factor for the consequent cellular response to radiation. DNA plasmid pBR322 was irradiated at U120-M cyclotron with 30 MeV protons and treated with two Escherichia coli base excision repair enzymes. The yields of SSBs and DSBs were analysed using agarose gel electrophoresis. DNA has been irradiated in the presence of hydroxyl radical scavenger (coumarin-3-carboxylic acid) in order to distinguish between direct and indirect damage of the biological target. Pure scavenger solution was used as a probe for measurement of induced OH center dot radical yields. Experimental OH center dot radical yield kinetics was compared with predictions computed by two theoretical models-RADAMOL and Geant4-DNA. Both approaches use Geant4-DNA for description of physical stages of radiation action, and then each of them applies a distinct model for description of the pre-chemical and chemical stage.

Författare

Katerina Pachnerova Brabcová

Chalmers, Teknisk fysik, Nukleär teknik

V. Stepan

Czech Academy of Sciences

Centre d'Etudes Nucleaires de Bordeaux Gradignan

M. Karamitros

Centre d'Etudes Nucleaires de Bordeaux Gradignan

M. Karabin

University of Chemistry and Technology, Prague

P. Dostalek

University of Chemistry and Technology, Prague

S. Incerti

Centre d'Etudes Nucleaires de Bordeaux Gradignan

M. Davidkova

Czech Academy of Sciences

Lembit Sihver

Chalmers, Teknisk fysik, Nukleär teknik

Radiation Protection Dosimetry

0144-8420 (ISSN)

Vol. 166 44-48

Ämneskategorier

Subatomär fysik

DOI

10.1093/rpd/ncv159