Normalization of Host Intestinal Mucus Layers Requires Long-Term Microbial Colonization
Artikel i vetenskaplig tidskrift, 2015

The intestinal mucus layer provides a barrier limiting bacterial contact with the underlying epithelium. Mucus structure is shaped by intestinal location and the microbiota. To understand how commensals modulate gut mucus, we examined mucus properties under germ-free (GF) conditions and during microbial colonization. Although the colon mucus organization of GF mice was similar to that of conventionally raised (Convr) mice, the GF inner mucus layer was penetrable to bacteria-sized beads. During colonization, in which GF mice were gavaged with Convr microbiota, the small intestine mucus required 5 weeks to be normally detached and colonic inner mucus 6 weeks to become impenetrable. The composition of the small intestinal microbiota during colonization was similar to Convr donors until 3 weeks, when Bacteroides increased, Firmicutes decreased, and segmented filamentous bacteria became undetectable. These findings highlight the dynamics of mucus layer development and indicate that studies of mature microbe-mucus interactions should be conducted weeks after colonization.

model

secretion

mucin

immune

homeostasis

bacteria

glycosylation

gut microbiota

mouse stomach

ecosystem

functional cftr channel

Författare

Malin E V Johansson

Göteborgs universitet

Hedvig E Jakobsson

Göteborgs universitet

Jessica Holmén Larsson

Göteborgs universitet

André Schütte

Göteborgs universitet

Anna Ermund

Göteborgs universitet

Ana María Rodríguez-Piñeiro

Göteborgs universitet

Liisa Arike

Göteborgs universitet

Catharina Wising

Göteborgs universitet

Frida Svensson

Göteborgs universitet

Fredrik Bäckhed

Göteborgs universitet

Gunnar C. Hansson

Göteborgs universitet

Cell Host and Microbe

1931-3128 (ISSN)

Vol. 18 5 582-592

Ämneskategorier

Mikrobiologi

DOI

10.1016/j.chom.2015.10.007