The Fun30 Chromatin Remodeler Fft3 Controls Nuclear Organization and Chromatin Structure of Insulators and Subtelomeres in Fission Yeast
Artikel i vetenskaplig tidskrift, 2015

In eukaryotic cells, local chromatin structure and chromatin organization in the nucleus both influence transcriptional regulation. At the local level, the Fun30 chromatin remodeler Fft3 is essential for maintaining proper chromatin structure at centromeres and subtelomeres in fission yeast. Using genome-wide mapping and live cell imaging, we show that this role is linked to controlling nuclear organization of its targets. In fft3? cells, subtelomeres lose their association with the LEM domain protein Man1 at the nuclear periphery and move to the interior of the nucleus. Furthermore, genes in these domains are upregulated and active chromatin marks increase. Fft3 is also enriched at retrotransposon-derived long terminal repeat (LTR) elements and at tRNA genes. In cells lacking Fft3, these sites lose their peripheral positioning and show reduced nucleosome occupancy. We propose that Fft3 has a global role in mediating association between specific chromatin domains and the nuclear envelope.


Babett Steglich

Karolinska Institutet

Annelie Strålfors

Karolinska Institutet

Olga Khorosjutina

Karolinska Institutet

Jenna Persson

Karolinska Institutet

Agata Smialowska

Chalmers, Biologi och bioteknik, Systembiologi

Karolinska Institutet

Jean Paul Javerzat

Centre national de la recherche scientifique (CNRS)

Institut Biochimie et Genetique Cellulaires, Bordeaux

Karl Ekwall

Karolinska Institutet

PLoS Genetics

1553-7390 (ISSN) 1553-7404 (eISSN)

Vol. 11 3 e1005101-


Biokemi och molekylärbiologi


Livsvetenskaper och teknik (2010-2018)



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