Identification of geranylgeranyl diphosphate synthase genes from Tripterygium wilfordii
Artikel i vetenskaplig tidskrift, 2015

We found triptolide synthesis is correlated with the expressions of TwGGPPS1 and TwGGPPS4 . This lays the foundation for future studies of biosynthetic pathways for triptolide and other diterpenoids in T. wilfordii. Tripterygium wilfordii is a traditional Chinese medical plant commonly used to treat rheumatoid arthritis. One of its main bioactive compounds is triptolide, which is identified as an abietane-type diterpenoid natural product. Geranylgeranyl diphosphate synthase (GGPPS) catalyses the synthesis of GGPP (geranylgeranyl diphosphate), the common precursor of diterpenes, and is therefore a crucial enzyme in diterpene biosynthesis. A previous study showed that GGPP could be catalyzed by copalyl diphosphate synthase and kaurene synthase like of Salvia miltiorrhiza (SmCPS, SmKSL) to miltiradiene, a key intermediate in tanshinone biosynthesis. In this paper, five new full-length cDNAs (TwGGPPS) encoding GGPP synthases were cloned from T. wilfordii. Sequence comparisons revealed that all six TwGGPPSs (including TwGGPPS2 cloned previously) exhibit similarities to GGPPSs of other plants. Subsequent functional complement assays demonstrated that TwGGPPS1, TwGGPPS4 and TwGGPPS5 can participate in miltiradiene biosynthesis in the recombinant E. coli. Correlation analysis of gene expressions and secondary metabolite accumulation indicated that TwGGPPS1 and TwGGPPS4 are likely involved in the biosynthesis of triptolide. These findings lay the foundation for future studies of the biosynthetic pathways for triptolide and other diterpenoids in T. wilfordii.

Tripterygium wilfordii

GGPPS

Biosynthesis

Triptolide

Författare

M. Zhang

Capital Medical University China

P. Su

China Academy of Chinese Medical Sciences

Capital Medical University China

Yongjin Zhou

Chalmers, Biologi och bioteknik, Systembiologi

X. Wang

Capital Medical University China

Y. J. Zhao

China Academy of Chinese Medical Sciences

Y. J. Liu

Capital Medical University China

Y. R. Tong

China Academy of Chinese Medical Sciences

Capital Medical University China

T. Y. Hu

Capital Medical University China

L. Q. Huang

China Academy of Chinese Medical Sciences

W. Gao

Capital Medical University China

Plant Cell Reports

0721-7714 (ISSN) 1432-203X (eISSN)

Vol. 34 2179-2188

Ämneskategorier

Bioinformatik och systembiologi

DOI

10.1007/s00299-015-1860-3