Analysis of neuropeptides by MALDI imaging mass spectrometry.
Reviewartikel, 2013

Matrix-assisted laser desorption ionization (MALDI) imaging mass spectrometry (IMS) is one of the most effective tools for localizing small molecules and compounds directly in thin tissue sections. MALDI IMS should be used when the distribution of molecular species is not known and to localize changes due to a disease process or a treatment. In recent years it has become increasingly clear that many pathological processes are not readily correlated to dramatic changes in protein levels. MALDI IMS can aid the localization of areas where the cellular concentration of proteins may be high enough to play an important biological role, but when the precise location is unknown. Here, we present a MALDI IMS protocol and data analysis of molecular imaging of multiple rat brain sections.

methods

Animals

Mass

Matrix-Assisted Laser Desorption-Ionization

methods

analysis

Brain Chemistry

Spectrometry

Rats

Neuropeptides

Diagnostic Imaging

Författare

Anna Ljungdahl

Jörg Hanrieder

Jonas Bergquist

Malin Andersson

Methods in molecular biology (Clifton, N.J.)

1940-6029 (ISSN)

Vol. 1023 121-36

Ämneskategorier

Medicinska grundvetenskaper

Neurovetenskaper

DOI

10.1007/978-1-4614-7209-4_7

PubMed

23765622