Proteins in aqueous humor from cataract patients with and without pseudoexfoliation syndrome.
Artikel i vetenskaplig tidskrift, 2012
The aim of this study was to investigate the protein content in aqueous humor in eyes with and without pseudoexfoliations (PEX) and to evaluate the quantitative proteomics method, isobaric tagging for relative and absolute protein quantification (iTRAQ), in combination with two separation methods followed by matrix-assisted Laser desorption/ionization (MALDI) mass spectrometry and tandem mass spectrometry (MS/MS). During cataract surgery, samples of aqueous humor were collected from 20 eyes with PEX and from 18 control eyes. The relative concentrations of proteins in the pooled samples of ten PEX eyes and eight controls were evaluated after trypsin digestion and Labeling of the peptides with (iTRAQ) reagent. Two separation methods, Liquid chromatography (LC) and capillary electrophoresis (CE) were used, followed by MALDI mass spectrometry and MS/MS. Furthermore, 1D gel electrophoresis was performed on the remaining ten pooled PEX samples and ten control samples. The gel material was separated by nano-liquid chromatography (nano-LC) followed by Linear-ion-trap quadrupole Fourier transformation ion cyclotron resonance (FT-ICR). Fifty four proteins were identified in the LC runs and 24 with CE. The relative concentrations of beta-crystallines B2 and S were raised and those of angiotensinogen and osteopontin Lowered in the PEX sample compared to the control. The trends regarding beta-crystallines B2, angiotensinogen and osteopontin were confirmed by the 1D gel electrophoresis.
Angiotensinogen
complications
chemistry
Cataract
analysis
Proteomics
metabolism
Aged
Aqueous Humor
analysis
complications
Female
metabolism
Proteins
metabolism
metabolism
Matrix-Assisted Laser Desorption-Ionization
Osteopontin
beta-Crystallins
analysis
Spectrometry
Male
Exfoliation Syndrome
Mass
analysis
metabolism
80 and over
Aged
metabolism
Humans
Författare
Amelie Botling Taube
Emilia Hardenborg
Magnus Wetterhall
Konstantin Artemenko
Jörg Hanrieder
Marit Andersson
Albert Alm
Jonas Bergquist
European Journal of Mass Spectrometry
1469-0667 (ISSN)
Vol. 18 6 531-41Ämneskategorier
Analytisk kemi
Oftalmologi
PubMed
23654198