MALDI mass spectrometry based molecular phenotyping of CNS glial cells for prediction in mammalian brain tissue.
Artikel i vetenskaplig tidskrift, 2011

The development of powerful analytical techniques for specific molecular characterization of neural cell types is of central relevance in neuroscience research for elucidating cellular functions in the central nervous system (CNS). This study examines the use of differential protein expression profiling of mammalian neural cells using direct analysis by means of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). MALDI-MS analysis is rapid, sensitive, robust, and specific for large biomolecules in complex matrices. Here, we describe a newly developed and straightforward methodology for direct characterization of rodent CNS glial cells using MALDI-MS-based intact cell mass spectrometry (ICMS). This molecular phenotyping approach enables monitoring of cell growth stages, (stem) cell differentiation, as well as probing cellular responses towards different stimulations. Glial cells were separated into pure astroglial, microglial, and oligodendroglial cell cultures. The intact cell suspensions were then analyzed directly by MALDI-TOF-MS, resulting in characteristic mass spectra profiles that discriminated glial cell types using principal component analysis. Complementary proteomic experiments revealed the identity of these signature proteins that were predominantly expressed in the different glial cell types, including histone H4 for oligodendrocytes and S100-A10 for astrocytes. MALDI imaging MS was performed, and signature masses were employed as molecular tracers for prediction of oligodendroglial and astroglial localization in brain tissue. The different cell type specific protein distributions in tissue were validated using immunohistochemistry. ICMS of intact neuroglia is a simple and straightforward approach for characterization and discrimination of different cell types with molecular specificity.

Proteins

Rats

ultrastructure

methods

cytology

ultrastructure

methods

chemistry

Spectrometry

cytology

analysis

Histones

analysis

Proteomics

Cells

Matrix-Assisted Laser Desorption-Ionization

Animals

Sprague-Dawley

Mass

Cultured

Neuroglia

Rats

Brain

Författare

Jörg Hanrieder

Göteborgs universitet

Grzegorz Wicher

Jonas Bergquist

Malin Andersson

Asa Fex-Svenningsen

Analytical and Bioanalytical Chemistry

1618-2642 (ISSN) 1618-2650 (eISSN)

Vol. 401 1 135-47

Ämneskategorier

Neurovetenskaper

Cell- och molekylärbiologi

DOI

10.1007/s00216-011-5043-y

PubMed

21553124

Mer information

Skapat

2017-10-10