Clinical perspectives of high-resolution mass spectrometry-based proteomics in neuroscience: exemplified in amyotrophic lateral sclerosis biomarker discovery research.
Reviewartikel, 2008

Biomarker discovery is a central application in today's proteomic research. There is an urgent need for valid biomarkers to improve diagnostic tools and treatment in many disorders, such as the rapidly progressing neurodegenerative disorder amyotrophic lateral sclerosis (ALS) that has a fatal outcome in about 3 years and yet no curative treatment. Screening for clinically relevant biomarkers puts high demands on high-throughput, rapid and precise proteomic techniques. There is a large variety in the methods of choice involving mainly gel-based approaches as well as chromatographic techniques for multi-dimensional protein and peptide separations followed by mass spectrometry (MS) analysis. This special feature article will discuss some important aspects of MS-based clinical proteomics and biomarker discovery in the field of neurodegenerative diseases and ALS research respectively, with the aim to provide a prospective view on current and future research aspects in the field. Furthermore, examples for application of high-resolution MS-based proteomic strategies for ALS biomarker discovery will be demonstrated with two studies previously reported by our group. These studies include among others, utilization of capillary liquid chromatography-Fourier transform ion cyclotron resonance mass spectrometry (LC-FTICR-MS) for advanced protein pattern classification in cerebrospinal fluid (CSF) samples of ALS patients as well as highly sensitive protein identification in minimal amounts of postmortem spinal cord tissue and laser micro-dissected motor neurons using FT-ICR-MS in conjunction with nanoflow LC coupled to matrix-assisted laser desorption ionization time-of-flight tandem mass spectrometry (LC-MALDI-TOF-TOF-MS).

Mass Spectrometry

Neurosciences

trends

Amyotrophic Lateral Sclerosis

analysis

Proteomics

analysis

metabolism

Biomarkers

diagnosis

Nerve Tissue Proteins

Humans

Brain

Proteome

analysis

metabolism

trends

trends

Författare

Titti Ekegren

Jörg Hanrieder

Göteborgs universitet

Jonas Bergquist

Journal of Mass Spectrometry

1076-5174 (ISSN)

Vol. 43 5 559-71

Ämneskategorier

Analytisk kemi

Annan klinisk medicin

Neurovetenskaper

Biologiska vetenskaper

Neurologi

DOI

10.1002/jms.1409

PubMed

18416436