Focused proteomics in post-mortem human spinal cord.
Artikel i vetenskaplig tidskrift, 2006

With a highly sensitive electrospray ionization-Fourier transform ion cyclotron resonance mass spectrometry (ESI-FTICR MS) system, proteins were identified in minimal amounts of spinal cord from patients with the neurodegenerative disease amyotrophic lateral sclerosis (ALS) and compared to proteins in spinal cord from control subjects. The results show 18 versus 16 significantly identified (p < 0.05) proteins, respectively, all known to be found in the central nervous system. The most abundant protein in both groups was the glial fibrillary acidic protein, GFAP. Other proteins were, for example, hemoglobin alpha- and beta chain, myelin basic protein, thioredoxin, alpha enolase, and choline acetyltransferase. This study also includes the technique of laser microdissection in combination with pressure catapulting (LMPC) for the dissection of samples and specific neurons. Furthermore, complementary experiments with nanoLC-matrix assisted laser desorption ionization time-of-flight tandem mass spectrometry (MALDI-TOF-TOF MS) confirmed the results of the ESI-FTICR MS screening and provided additional results of further identified proteins.

chemistry

metabolism

methods

Humans

Proteomics

Mass

Dissection

Amyotrophic Lateral Sclerosis

Spinal Cord

analysis

Lasers

Postmortem Changes

Electrospray Ionization

Proteins

Spectrometry

methods

Författare

Titti Ekegren

Jörg Hanrieder

Göteborgs universitet

Sten-Magnus Aquilonius

Jonas Bergquist

Journal of Proteome Research

1535-3893 (ISSN) 1535-3907 (eISSN)

Vol. 5 2364-71

Ämneskategorier

Neurovetenskaper

Neurologi

DOI

10.1021/pr060237f

PubMed

16944948