The molecular basis of polysaccharide cleavage by lytic polysaccharide monooxygenases
Artikel i vetenskaplig tidskrift, 2016

Lytic polysaccharide monooxygenases (LPMOs) are copper-containing enzymes that oxidatively break down recalcitrant polysaccharides such as cellulose and chitin. Since their discovery, LPMOs have become integral factors in the industrial utilization of biomass, especially in the sustainable generation of cellulosic bioethanol. We report here a structural determination of an LPMO-oligosaccharide complex, yielding detailed insights into the mechanism of action of these enzymes. Using a combination of structure and electron paramagnetic resonance spectroscopy, we reveal the means by which LPMOs interact with saccharide substrates. We further uncover electronic and structural features of the enzyme active site, showing how LPMOs orchestrate the reaction of oxygen with polysaccharide chains.

Författare

K. E. H. Frandsen

Köbenhavns Universitet

T. J. Simmons

University of Cambridge

P. Dupree

University of Cambridge

J. C. N. Poulsen

Köbenhavns Universitet

G. R. Hemsworth

University of York

L. Ciano

University of York

E. M. Johnston

University of York

M. Tovborg

Novozymes A/S

Katja Salomon Johansen

Chalmers, Biologi och bioteknik, Industriell bioteknik

P. von Freiesleben

Novozymes A/S

L. Marmuse

Université Grenoble Alpes

S. Fort

Université Grenoble Alpes

S. Cottaz

Université Grenoble Alpes

H. Driguez

Université Grenoble Alpes

B. Henrissat

King Abdulaziz University

INRA Avignon

Aix Marseille Université

N. Lenfant

Aix Marseille Université

INRA Avignon

F. Tuna

University of Manchester

A. Baldansuren

University of Manchester

G. J. Davies

University of York

L. Lo Leggio

Köbenhavns Universitet

P. H. Walton

University of York

Nature Chemical Biology

1552-4450 (ISSN) 1552-4469 (eISSN)

Vol. 12 298-+

Ämneskategorier

Kemiteknik

DOI

10.1038/nchembio.2029