Metabolic engineering of isoprenoid production: Reconstruction of multistep heterologous pathways in tractable hosts
Kapitel i bok, 2013

Isoprenoids represent a wide group of chemically active compounds that can find a wide range of applications as flavors, perfumes, vitamins, nutraceuticals, and pharmaceuticals. Many isoprenoids are naturally produced in very low quantities by plants, which make their use in broader perspectives difficult. Microbial production of plant-originating isoprenoids quickly appeared as an alternative of choice. Metabolic engineering methods were applied and proved successful to improve the supply of precursors to derive toward isoprenoid compounds of interest. Combinations between metabolic engineering and the flourishing field of synthetic biology have also been observed with researchers attempting to reconstruct and optimize complex biosynthetic pathways in well-characterized and tractable microbial hosts. In this chapter, we review recent metabolic engineering studies for isoprenoid production in yeast and try to show, through key examples, that the fields of synthetic biology and metabolic engineering can go hand in hand to establish microbial cell factories for isoprenoid production.

MEP pathway

Heterologous gene

Synthetic biology

Metabolic engineering

Mevalonate pathway

Yeast

Saccharomyces cerevisiae

Författare

J. Maury

Danmarks Tekniske Universitet (DTU)

M. A. Asadollahi

Danmarks Tekniske Universitet (DTU)

L. R. Formenti

Danmarks Tekniske Universitet (DTU)

S. Michel

Firmenich Inc.

Jens B Nielsen

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

Isoprenoid Synthesis in Plants and Microorganisms: New Concepts and Experimental Approaches

73-89

Ämneskategorier

Bioinformatik och systembiologi

DOI

10.1007/978-1-4614-4063-5_6

ISBN

9781461440635

Mer information

Senast uppdaterat

2018-09-06