A multi-purpose microfluidic pipette for single-cell analysis
Paper i proceeding, 2010

We report a multi-purpose microfluidic pipette, with a recirculating liquid tip. This device, made in poly(dimethylsiloxane), enables contamination-free manipulation and chemical stimulation of selected single cells in cell collectives or tissue slices. The pipette is capable of carrying out a variety of complex fluid processing functionalities, such as mixing, multiplexing, or gradient generation. The concept is flexible and scalable as the geometry and the size of the recirculation zone is defined by pressure, channel number, and geometry. We have applied the pipette in a fluorescence uptake assay, electrophysiology studies and for chemical induction of membrane protrusion from biological cells.

Chemical induction

Solution exchange

Single cells

Liquids

Chemical stimulation

Superfusion

Single-cell manipulation

Singlecell analysis

Microfluidics

Contamination-free

Recirculation zones

Molecular biology

Electrophysiology

Single-cell analysis

Electrophysiology study

Författare

Alar Ainla

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Erik Jansson

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Natalia Stepanyants

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Owe Orwar

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Aldo Jesorka

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010; Groningen; Netherlands; 3 October 2010 through 7 October 2010

Vol. 2 932-934

Ämneskategorier

Fysikalisk kemi

ISBN

9781618390622