Proteomic Analysis of Early Mid-Trimester Amniotic Fluid Does Not Predict Spontaneous Preterm Delivery
Artikel i vetenskaplig tidskrift, 2016

Objective The aim of this study was to identify early proteomic biomarkers of spontaneous preterm delivery (PTD) in mid-trimester amniotic fluid from asymptomatic women. This is a case-cohort study. Amniotic fluid from mid-trimester genetic amniocentesis (14-19 weeks of gestation) was collected from 2008 to 2011. The analysis was conducted in 24 healthy women with subsequent spontaneous PTD (cases) and 40 randomly selected healthy women delivering at term (controls). An exploratory phase with proteomics analysis of pooled samples was followed by a verification phase with ELISA of individual case and control samples. The median (interquartile range (IQR: 25th; 75th percentiles) gestational age at delivery was 35+5 (33+6-36+6) weeks in women with spontaneous PTD and 40+0 (39+1-40+5) weeks in women who delivered at term. In the exploratory phase, the most pronounced differences were found in C-reactive protein (CRP) levels, that were approximately two-fold higher in the pooled case samples than in the pooled control samples. However, we could not verify these differences with ELISA. The median (25th; 75th IQR) CRP level was 95.2 ng/mL (64.3; 163.5) in women with spontaneous PTD and 86.0 ng/mL (51.2; 145.8) in women delivering at term (p = 0.37; t-test). Proteomic analysis with mass spectrometry of mid-trimester amniotic fluid suggests CRP as a potential marker of spontaneous preterm delivery, but this prognostic potential was not verified with ELISA.

Författare

Maria Hallingström

Göteborgs universitet

J. Lenco

University of Defence in Brno Faculty of Military Health Sciences

M. Vajrychova

University of Defence in Brno Faculty of Military Health Sciences

M. Link

University of Defence in Brno Faculty of Military Health Sciences

V. Tambor

Faculty Hospital at Hradec Kralove

V. Liman

Sahlgrenska universitetssjukhuset

Maria Bullarbo

Göteborgs universitet

Staffan Nilsson

Chalmers, Matematiska vetenskaper, matematisk statistik

Göteborgs universitet

Panos Tsiartas

Göteborgs universitet

T. Cobo

CIBER Enfermedades Raras

Sahlgrenska universitetssjukhuset

Universitat de Barcelona

M. Kacerovsky

Univerzita Karlova v Praze

Faculty Hospital at Hradec Kralove

Bo Jacobsson

Göteborgs universitet

PLoS ONE

1932-6203 (ISSN)

Vol. 11 5 article no. e0155164- e0155164

Ämneskategorier

Reproduktionsmedicin och gynekologi

DOI

10.1371/journal.pone.0155164

PubMed

27214132