Dynamic and Progressive Control of DNA Origami Conformation by Modulating DNA Helicity with Chemical Adducts
Artikel i vetenskaplig tidskrift, 2016

DNA origami has received enormous attention for its ability to program complex nanostructures with a few nanometer precision. Dynamic origami structures that change conformation in response to environmental cues or external signals hold great promises in sensing and actuation at the nanoscale. The reconfiguration mechanism of existing dynamic origami structures is mostly limited to single-stranded hinges and relies almost exclusively on DNA hybridization or strand displacement. Here, we show an alternative approach by demonstrating on-demand conformation changes with DNA-binding molecules, which intercalate between base pairs and unwind DNA double helices. The unwinding effect modulates the helicity mismatch in DNA origami, which significantly influences the internal stress and the global conformation of the origami structure. We demonstrate the switching of a polymerized origami nanoribbon between different twisting states and a well-constrained torsional deformation in a monomeric origami shaft. The structural transformation is shown to be reversible, and binding isotherms confirm the reconfiguration mechanism. This approach provides a rapid and reversible means to change DNA origami conformation, which can be used for dynamic and progressive control at the nanoscale.

conformation change

DNA binding

DNA origami

Författare

H. R. Chen

Purdue University

H. Y. Zhang

Purdue University

J. Pan

Purdue University

T. G. Cha

Purdue University

Shiming Li

Chalmers, Kemi och kemiteknik, Kemi och biokemi

Joakim Andreasson

Chalmers, Kemi och kemiteknik, Kemi och biokemi

J. H. Choi

Purdue University

ACS Nano

1936-0851 (ISSN) 1936-086X (eISSN)

Vol. 10 5 4989-4996

Styrkeområden

Nanovetenskap och nanoteknik

Ämneskategorier

Fysikalisk kemi

DOI

10.1021/acsnano.6b01339

Mer information

Senast uppdaterat

2020-08-31