Cellular uptake of PLGA nanoparticles targeted with anti-amyloid and anti-transferrin receptor antibodies for Alzheimer's disease treatment
Artikel i vetenskaplig tidskrift, 2016

During the last few decades, relevant efforts have been reported to design nanocarriers for drug transport through the blood brain barrier (BBB). New drugs, such as peptide iA?5, capable to inhibit the aggregates associated with Alzheime?s disease (AD) are being tested but the most frequent drawback is to reach the brain in the desired concentrations due to the low BBB permeability-surface area. Our approach, as a proof of concept to improve drug transport through the BBB, is based on poly(lactic-co-glycolic acid) (PLGA) nanoparticles with surface functionalized with anti-transferrin receptor monoclonal antibody (OX26) and anti-A? (DE2B4) to deliver encapsulated iA?5 into the brain. Porcine brain capillary endothelial cells (PBCECs) were used as a BBB model to evaluate the system efficacy and toxicity. The uptake of immune nanoparticles with a controlled delivery of the peptide iA?5 was substantially increased compared to the nanoparticles (NPs) without monoclonal antibody functionalization.

Drug delivery systems

Alzheimer's disease

Immuno nanoparticles

Antibodies

Blood brain-barrier

Therapeutic peptides

Författare

J. A. Loureiro

Universidade do Porto

Bárbara Gomes

Universidade do Porto

Gert Fricker

Universitat Heidelberg

M. A. N. Coelho

Universidade do Porto

Sandra Rocha

Chalmers, Biologi och bioteknik, Kemisk biologi

M. C. Pereira

Universidade do Porto

Colloids and Surfaces B: Biointerfaces

0927-7765 (ISSN)

Vol. 145 8-13

Ämneskategorier

Kemiteknik

DOI

10.1016/j.colsurfb.2016.04.041