Influence of Divalent Cations on Deformation and Rupture of Adsorbed Lipid Vesicles
Artikel i vetenskaplig tidskrift, 2016

The fate of adsorbed lipid vesicles on solid supports depends on numerous experimental parameters and typically results in the formation of a supported lipid bilayer (SLB) or an adsorbed vesicle layer. One of the poorly understood questions relates to how divalent cations appear to promote SLB formation in some cases. The complexity arises from the multiple ways in which divalent cations affect vesicle substrate and vesicle vesicle interactions as well as vesicle properties. These interactions are reflected, e.g., in the degree of deformation of adsorbed vesicles (if they do not rupture). It is, however, experimentally challenging to measure the extent of vesicle deformation in real-time. Herein, we investigated the effect of divalent cations (Mg2+, Ca2+, Sr2+) on the adsorption of zwitterionic 1,2-dioleoylsn-glycero-3-phosphocholine (DOPC) lipid vesicles onto silicon oxide- and titanium oxide coated substrates. The vesicle adsorption process was tracked using the quartz crystal microbalance-dissipation (QCM-D) and localized surface plasmon resonance (LSPR) measurement techniques. On silicon oxide, vesicle adsorption led to SLB formation in all cases, while vesicles adsorbed but did not rupture on titanium oxide. It was identified that divalent cations promote increased deformation of adsorbed vesicles on both substrates and enhanced rupture on silicon oxide in the order Ca2+ > Mg2+ > Sr2+. The influence of divalent cations on different factors in these systems is discussed, clarifying experimental observations on both substrates. Taken together, the findings in this work offer insight into how divalent cations modulate the interfacial science of supported membrane systems.

glass

adsorption

unilamellar

Chemistry

supported phospholipid-bilayers

titanium-dioxide

membrane

surface-plasmon resonance

quartz-crystal microbalance

Materials Science

ion-binding

vesicles

atomic-force microscopy

Författare

M. Dacic

Nanyang Technological University

J. Jackman

Nanyang Technological University

S. Yorulmaz

Nanyang Technological University

V. P. Zhdanov

Nanyang Technological University

Russian Academy of Sciences

Bengt Herbert Kasemo

Chalmers, Fysik, Kemisk fysik

N. J. Cho

Nanyang Technological University

Langmuir

0743-7463 (ISSN) 1520-5827 (eISSN)

Vol. 32 25 6486-6495

Ämneskategorier

Materialteknik

DOI

10.1021/acs.langmuir.6b00439

PubMed

27182843