Hydrodynamic Propulsion of Liposomes Electrostatically Attracted to a Lipid Membrane Reveals Size-Dependent Conformational Changes
Artikel i vetenskaplig tidskrift, 2016

The efficiency of lipid nanoparticle uptake across cellular membranes is strongly dependent on the very first interaction step. Detailed understanding of this step is in part hampered by the large heterogeneity in the physicochemical properties of lipid nanoparticles, such as liposomes, making conventional ensemble-averaging methods too blunt to address details of this complex process. Here, we contribute a means to explore whether individual liposomes become deformed upon binding to fluid cell-membrane mimics. This was accomplished by using hydrodynamic forces to control the propulsion of nanoscale liposomes electrostatically attracted to a supported lipid bilayer. In this way, the size of individual liposomes could be determined by simultaneously measuring both their individual drift velocity and diffusivity, revealing that for a radius of similar to 45 nm, a close agreement with dynamic light scattering data was observed, while larger liposomes (radius similar to 75 nm) displayed a significant deformation unless composed of a gel-phase lipid. The relevance of being able to extract this type of information is discussed in the context of membrane fusion and cellular uptake.

shear-flow

Chemistry

chemistry and physics of lipids

supported bilayer

single-particle tracking

deformation

single particle tracking

supported lipid bilayer

synaptic vesicle fusion

1993

cells

bilayer formation

Materials Science

nanoparticles

p187

hydrodynamic

gier j

optical measurements

liposome deformation

curvature

Science & Technology - Other Topics

TIRF microscopy

v64

Författare

Seyed Tabaei

Nanyang Technological University

J. J. J. Gillissen

Nanyang Technological University

Stephan Block

Chalmers, Fysik, Biologisk fysik

Fredrik Höök

Chalmers, Fysik, Biologisk fysik

N. J. Cho

Nanyang Technological University

ACS Nano

1936-0851 (ISSN) 1936-086X (eISSN)

Vol. 10 8812-8820

Ämneskategorier

Fysik

DOI

10.1021/acsnano.6b04572