Hydrodynamic Propulsion of Liposomes Electrostatically Attracted to a Lipid Membrane Reveals Size-Dependent Conformational Changes
Artikel i vetenskaplig tidskrift, 2016
The efficiency of lipid nanoparticle uptake across cellular membranes is strongly dependent on the very first interaction step. Detailed understanding of this step is in part hampered by the large heterogeneity in the physicochemical properties of lipid nanoparticles, such as liposomes, making conventional ensemble-averaging methods too blunt to address details of this complex process. Here, we contribute a means to explore whether individual liposomes become deformed upon binding to fluid cell-membrane mimics. This was accomplished by using hydrodynamic forces to control the propulsion of nanoscale liposomes electrostatically attracted to a supported lipid bilayer. In this way, the size of individual liposomes could be determined by simultaneously measuring both their individual drift velocity and diffusivity, revealing that for a radius of similar to 45 nm, a close agreement with dynamic light scattering data was observed, while larger liposomes (radius similar to 75 nm) displayed a significant deformation unless composed of a gel-phase lipid. The relevance of being able to extract this type of information is discussed in the context of membrane fusion and cellular uptake.
shear-flow
Chemistry
chemistry and physics of lipids
supported bilayer
single-particle tracking
deformation
single particle tracking
supported lipid bilayer
synaptic vesicle fusion
1993
cells
bilayer formation
Materials Science
nanoparticles
p187
hydrodynamic
gier j
optical measurements
liposome deformation
curvature
Science & Technology - Other Topics
TIRF microscopy
v64
Författare
Seyed Tabaei
Nanyang Technological University
J. J. J. Gillissen
Nanyang Technological University
Stephan Block
Chalmers, Fysik, Biologisk fysik
Fredrik Höök
Chalmers, Fysik, Biologisk fysik
N. J. Cho
Nanyang Technological University
ACS Nano
1936-0851 (ISSN) 1936-086X (eISSN)
Vol. 10 9 8812-8820Ämneskategorier
Fysik
DOI
10.1021/acsnano.6b04572