Non-targeted transcriptomic effects upon thyroid irradiation: similarity between in-field and out-of-field responses varies with tissue type
Artikel i vetenskaplig tidskrift, 2016

Non-targeted effects can induce responses in tissues that have not been exposed to ionizing radiation. Despite their relevance for risk assessment, few studies have investigated these effects in vivo. In particular, these effects have not been studied in context with thyroid exposure, which can occur e.g. during irradiation of head and neck tumors. To determine the similarity between in-field and out-offield responses in normal tissue, we used a partial body irradiation setup with female mice where the thyroid region, the thorax and abdomen, or all three regions were irradiated. After 24h, transcriptional regulation in the kidney cortex, kidney medulla, liver, lungs, spleen, and thyroid was analyzed using microarray technology. Thyroid irradiation resulted in transcriptional regulation in the kidney medulla and liver that resembled regulation upon direct exposure of these tissues regarding both strength of response and associated biological function. The kidney cortex showed fewer similarities between the setups, while the lungs and spleen showed little similarity between in-field and out-of-field responses. Interestingly, effects were generally not found to be additive. Future studies are needed to identify the molecular mechanisms that mediate these systemic effects, so that they may be used as targets to minimize detrimental side effects in radiotherapy.

Författare

Britta Langen

Göteborgs universitet

Chalmers, Fysik

Nils Rudqvist

Göteborgs universitet

Johan Spetz

Göteborgs universitet

Janos Swanpalmer

Göteborgs universitet

Khalil Helou

Göteborgs universitet

Eva Forssell-Aronsson

Göteborgs universitet

Scientific Reports

2045-2322 (ISSN)

Vol. 6 Article number: 30738- 30738

Ämneskategorier

Biokemi och molekylärbiologi

Bioinformatik och systembiologi

Radiologi och bildbehandling

DOI

10.1038/srep30738

PubMed

27779251