Lowered Expression of Tumor Suppressor Candidate MYO1C Stimulates Cell Proliferation, Suppresses Cell Adhesion and Activates AKT
Artikel i vetenskaplig tidskrift, 2016

Myosin-1C (MYO1C) is a tumor suppressor candidate located in a region of recurrent losses distal to TP53. Myo1c can tightly and specifically bind to PIP2, the substrate of Phosphoinositide 3-kinase (PI3K), and to Rictor, suggesting a role for MYO1C in the PI3K pathway. This study was designed to examine MYO1C expression status in a panel of well-stratified endometrial carcinomas as well as to assess the biological significance of MYO1C as a tumor suppressor in vitro. We found a significant correlation between the tumor stage and lowered expression of MYO1C in endometrial carcinoma samples. In cell transfection experiments, we found a negative correlation between MYO1C expression and cell proliferation, and MYO1C silencing resulted in diminished cell migration and adhesion. Cells expressing excess of MYO1C had low basal level of phosphorylated protein kinase B (PKB, a. k. a. AKT) and cells with knocked down MYO1C expression showed a quicker phosphorylated AKT (pAKT) response in reaction to serum stimulation. Taken together the present study gives further evidence for tumor suppressor activity of MYO1C and suggests MYO1C mediates its tumor suppressor function through inhibition of PI3K pathway and its involvement in loss of contact inhibition.

Författare

Kittichate Visuttijai

Sahlgrenska akademin

Högskolan i Skövde

J. Pettersson

Sahlgrenska akademin

Y. M. Azar

Högskolan i Skövde

I. van den Bout

Universiteit van Pretoria

C. Orndal

Sahlgrenska universitetssjukhuset

J. Marcickiewicz

Hallands Hospital Varberg

Staffan Nilsson

Chalmers, Matematiska vetenskaper, Tillämpad matematik och statistik

Göteborgs universitet

M. Hornquist

Linköpings universitet

B. Olsson

Högskolan i Skövde

K. Ejeskar

Högskolan i Skövde

Afrouz Behboudi

Högskolan i Skövde

PLoS ONE

1932-6203 (ISSN)

Vol. 11 10 e0164063- e0164063

Ämneskategorier

Biologiska vetenskaper

DOI

10.1371/journal.pone.0164063

Mer information

Senast uppdaterat

2018-04-17