Induction of Laccase, Lignin Peroxidase and Manganese Peroxidase Activities in White-Rot Fungi Using Copper Complexes
Artikel i vetenskaplig tidskrift, 2016

Ligninolytic enzymes, such as laccase, lignin peroxidase and manganese peroxidase, are biotechnologically-important enzymes. The ability of five white-rot fungal strains Daedaleopsis confragosa, Fomes fomentarius, Trametes gibbosa, Trametes suaveolens and Trametes versicolor to produce these enzymes has been studied. Three different copper(II) complexes have been prepared ((Him)[Cu(im)(4)(H2O)(2)](btc)center dot 3H(2)O, where im = imidazole, H(3)btc = 1,3,5-benzenetricarboxylic acid, [Cu-3(pmdien)(3)(btc)](ClO4)(3)center dot 6H(2)O) and [Cu-3(mdpta)(3)(btc)](ClO4)(3)center dot 4H(2)O, where pmdien = N,N,N',N", N"-pentamethyl-diethylenetriamine and mdpta = N,N-bis-(3-aminopropyl) methyl-amine), and their potential application for laccase and peroxidases induction have been tested. The enzyme-inducing activities of the complexes were compared with that of copper sulfate, and it has been found that all of the complexes are suitable for the induction of laccase and peroxidase activities in white-rot fungi; however, the newly-synthesized complex M1 showed the greatest potential for the induction. With respect to the different copper inducers, this parameter seems to be important for enzyme activity, which depends also on the fungal strains.

copper complex

peroxidase

enzymatic induction

trimesic acid

laccase

Författare

M. Vrsanska

Mendelova univerzita v Brne

S. Voberkova

Vysoke Uceni Technicke v Brne, Stredoevropsku Technologicku Institut

Mendelova univerzita v Brne

Vratislav Langer

Chalmers, Kemi och kemiteknik, Energi och material, Oorganisk miljökemi

D. Palovcikova

Mendelova univerzita v Brne

A. Moulick

Vysoke Uceni Technicke v Brne, Stredoevropsku Technologicku Institut

Mendelova univerzita v Brne

V. Adam

Mendelova univerzita v Brne

Vysoke Uceni Technicke v Brne, Stredoevropsku Technologicku Institut

Pavel Kopel

Vysoke Uceni Technicke v Brne, Stredoevropsku Technologicku Institut

Mendelova univerzita v Brne

Molecules

1420-3049 (ISSN)

Vol. 21 11 1553

Ämneskategorier

Organisk kemi

DOI

10.3390/molecules21111553