Ferritin-supported lipid bilayers for triggering the endothelial cell response
Artikel i vetenskaplig tidskrift, 2017

Hybrid nanoassemblies of ferritin and silica-supported lipid bilayers (ferritin-SLBs) have been prepared and tested for the adhesion, spreading and proliferation of retinal microvascular endothelial cells (ECs). Lipid membranes with varying surface charge were obtained by mixing cationic 1-palmitoyl-2-oleoyl-sn-glycero-3-ethylphosphocholine (POEPC) with zwitterionic 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) at increasing POPC/POEPC ratios. The supported bilayer formation and their subsequent interaction processes with ferritin were studied at the pH of 7.4 at different protein concentrations, by using the quartz crystal microbalance with dissipation monitoring and by atomic force microscopy. Both kinetics and viscoelastic parameters of the protein-lipid membrane interface were scrutinized, as well as surface coverage. Phase-contrast optical microscopy analyses of the ferritin-SLBs substrates after their interaction with endothelial cells evidenced the highest cell adhesion (2-4 h of incubation time) and proliferation (from 24 h to 5 days) for the membranes of POPC/POEPC (75:25 ratio). Moreover, ferritin increased both cell adhesion and proliferation in comparison to control glass (respectively 1.5- and 1.75-fold) as well as proliferation in comparison to bare POPC/POEPC (95:5 ratio) (2 fold). Results are very promising in the goal of modulating the endothelial cell response through the interplay of viscoelastic/charge properties of the solid-supported membranes and the SLB-conditioned ferritin activity.

Protein-lipid membrane nanoassembly

QCM-D

Viscoelastic properties

AFM

Silica supported lipid membranes

Retinal microvascular endothelial cells

Författare

C. Satriano

Universita degli Studi di Catania

G. Lupo

Universita degli Studi di Catania

C. Motta

Universita degli Studi di Catania

C. D. Anfuso

Universita degli Studi di Catania

P. Di Pietro

Universita degli Studi di Catania

Bengt Herbert Kasemo

Chalmers, Fysik, Kemisk fysik

Colloids and Surfaces B: Biointerfaces

0927-7765 (ISSN)

Vol. 149 48-55

Ämneskategorier

Kemi

DOI

10.1016/j.colsurfb.2016.10.004